Human IL-6 QuantiGlo ELISA Kit

(14 citations)   
  • Assay Length
    5.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
  • Sensitivity
    0.35 pg/mL
  • Assay Range
    0.5 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-6
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The QuantiGlo Human IL-6 Chemiluminescent Immunoassay is a solid phase ELISA that specifically measures human IL-6 in less than 5.5 hours. This kit is designed to measure IL-6 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-6 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6. It has been observed in our laboratories that the measurement of human IL-6 is insensitive to the addition of the recombinant form of the human IL-6 soluble receptor.

Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.070.324.1537.


The recovery of IL-6 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-113
Citrate Plasma (n=4) 87 82-96
EDTA Plasma (n=4) 89 82-95
Heparin Plasma (n=4) 88 80-97
Serum (n=4) 94 88-106
To assess the linearity of the assay, samples spiked with high concentrations of IL-6 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-6 QuantiGlo ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
    • Long Name
      Interleukin 6
    • Entrez Gene IDs
      3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit);
    • Alternate Names
      B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta 2; IFN-beta-2; IL6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A;
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 14
    Filter your results:

    Sample Type
    1. A pilot study of telmisartan for visceral adiposity in HIV infection: the metabolic abnormalities, telmisartan, and HIV infection (MATH) trial.
      Authors: Lake J, Tseng C, Currier J
      PLoS ONE, 2013;8(3):e58135.
      Species: Human
      Sample Type: Serum
    2. Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue.
      Authors: Cifuentes M, Fuentes C, Tobar N, Acevedo I, Villalobos E, Hugo E, Ben-Jonathan N, Reyes M
      Mol Cell Endocrinol, 2012;361(1):24-30.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Angiotensin converting enzyme inhibitor and HMG-CoA reductase inhibitor as adjunct treatment for persons with HIV infection: a feasibility randomized trial.
      Authors: Baker J, Huppler Hullsiek K, Prosser R, Duprez D, Grimm R, Tracy R, Rhame F, Henry K, Neaton J
      PLoS ONE, 2012;7(10):e46894.
      Species: Human
      Sample Type: Plasma
    4. Inflammation, coagulation and cardiovascular disease in HIV-infected individuals.
      Authors: Duprez, Daniel A, Neuhaus, Jacqueli, Kuller, Lewis H, Tracy, Russell, Belloso, Waldo, De Wit, Stephane, Drummond, Fraser, Lane, H Cliffo, Ledergerber, Bruno, Lundgren, Jens, Nixon, Daniel, Paton, Nicholas, Prineas, Ronald J, Neaton, James D
      PLoS ONE, 2012;7(9):e44454.
      Species: Human
      Sample Type: Plasma
    5. Activation of nuclear factor-kappa B signalling promotes cellular senescence.
      Authors: Rovillain E, Mansfield L, Caetano C, Alvarez-Fernandez M, Caballero OL, Medema RH, Hummerich H, Jat PS
      Oncogene, 2011;30(20):2356-66.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Early inflammatory and metabolic changes in association with AGTR1 polymorphisms in prehypertensive subjects.
      Authors: Fung MM, Rao F, Poddar S
      Am. J. Hypertens., 2011;24(2):225-33.
      Species: Human
      Sample Type: Plasma
    7. Biomarkers of inflammation and development of rheumatoid arthritis in women from two prospective cohort studies.
      Authors: Karlson EW, Chibnik LB, Tworoger SS, Lee IM, Buring JE, Shadick NA, Manson JE, Costenbader KH
      Arthritis Rheum., 2009;60(3):641-52.
      Species: Human
      Sample Type: Plasma
    8. Obesity in pregnancy stimulates macrophage accumulation and inflammation in the placenta.
      Authors: Challier JC, Basu S, Bintein T, Minium J, Hotmire K, Catalano PM, Hauguel-De Mouzon S
      Placenta, 2008;29(3):274-81.
      Species: Human
      Sample Type: Plasma
    9. Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
      Authors: Fichorova RN, Richardson-Harman N, Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Curtis K, Dezzutti CS, Donoval B, Doncel GF, Donaghay M, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pasicznyk JM, Pallansch-Cokonis M, Poli G, Reichelderfer P, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R, Cummins JE
      Anal. Chem., 2008;80(12):4741-51.
      Species: Human
      Sample Type: WHO 89/548 reference standard
    10. Usefulness of C-reactive protein and interleukin-6 as predictors of outcomes in patients with chronic obstructive pulmonary disease receiving pravastatin.
      Authors: Lee TM, Lin MS, Chang NC
      Am. J. Cardiol., 2007;101(4):530-5.
      Species: Human
      Sample Type: Plasma
    11. Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes.
      Authors: Fritzenwanger M, Meusel K, Foerster M, Kuethe F, Krack A, Figulla HR
      Cytokine, 2007;38(3):137-44.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma.
      Authors: Feldmann G, Nischalke HD, Nattermann J, Banas B, Berg T, Teschendorf C, Schmiegel W, Duhrsen U, Halangk J, Iwan A, Sauerbruch T, Caselmann WH, Spengler U
      Clin. Cancer Res., 2006;12(15):4491-8.
      Species: Human
      Sample Type: Serum
    13. Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome in Nagano, Japan: clinical, radiological, and cytokine studies of 13 patients.
      Authors: Oide T, Ohara S, Oguchi K, Maruyama M, Yazawa M, Inoue K, Sekijima Y, Tokuda T, Ikeda S
      Clin. Exp. Rheumatol., 2004;22(1):91-8.
      Species: Human
      Sample Type: Serum
    14. Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients.
      Authors: Dahaba AA, Elawady GA, Rehak PH, List WF
      Anaesth Intensive Care, 2002;30(3):269-74.
      Species: Human
      Sample Type: Plasma
    Expand to show all
    ELISA Controls
    Description Application Cat# Citations Images  

    QuantiGlo Immunoassay Control Set 732 for Human IL-6

    Ctrl QC193
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Human IL-6 QuantiGlo ELISA Kit?

    Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest
    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed


    Customer Information