Human IL-6 QuantiGlo ELISA Kit

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Human IL-6 Chemiluminescent ELISA Standard Curve
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Product Details
Citations (39)
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Human IL-6 QuantiGlo ELISA Kit Summary

Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
0.35 pg/mL
Assay Range
0.5 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-6
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The QuantiGlo Human IL-6 Chemiluminescent Immunoassay is a solid phase ELISA that specifically measures human IL-6 in less than 5.5 hours. This kit is designed to measure IL-6 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-6 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6. It has been observed in our laboratories that the measurement of human IL-6 is insensitive to the addition of the recombinant form of the human IL-6 soluble receptor.

Precision

Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3 4
n 20 20 20 20 20 20 20 20
Mean (pg/mL) 1.21 10.7 118 766 1.56 13.2 142 752
Standard Deviation 0.07 0.32 4.15 37.5 0.15 1.02 8.93 53.7
CV% 5.8 3 3.5 4.9 9.6 7.7 6.3 7.1

Recovery

The recovery of IL-6 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-113
Citrate Plasma (n=4) 87 82-96
EDTA Plasma (n=4) 89 82-95
Heparin Plasma (n=4) 88 80-97
Serum (n=4) 94 88-106

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of IL-6 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-6 Chemiluminescent ELISA Linearity

Scientific Data

Human IL-6 Chemiluminescent ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells (2, 11-33). IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids (2). Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery (34-38). 

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (39). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (40). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130- expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 41). 
IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis (2, 5). IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity (1). It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis (1, 2). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of memory B cells and plasma cells (42).

Long Name:
Interleukin 6
Entrez Gene IDs:
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names:
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Citations for Human IL-6 QuantiGlo ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. A randomized phase 2 study on demeclocycline in patients with mild-to-moderate COVID-19
    Authors: Iwahori, K;Nii, T;Yamaguchi, N;Kawasaki, T;Okamura, S;Hashimoto, K;Matsuki, T;Tsujino, K;Miki, K;Osa, A;Goya, S;Abe, K;Mori, M;Takeda, Y;Yamada, T;Kida, H;Kumanogoh, A;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  2. Lidocaine attenuates hypoxia/reoxygenation?induced inflammation, apoptosis and ferroptosis in lung epithelial cells by regulating the p38 MAPK pathway
    Authors: X Ma, W Yan, N He
    Molecular Medicine Reports, 2022-03-04;25(5):.
    Species: Human
    Sample Types: cell culture supernatant
  3. The proton ATPase inhibitor bafilomycin A1 reduces the release of rhinovirus C and cytokines from primary cultures of human nasal epithelial cells
    Authors: M Yamaya, X Deng, A Kikuchi, M Sugawara, N Saito, T Kubo, H Momma, T Kawase, K Nakagome, Y Shimotai, H Nishimura
    Virus research, 2021-08-21;304(0):198548.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. miRNA-1246 in extracellular vesicles secreted from metastatic tumor induces drug resistance in tumor endothelial cells
    Authors: C Torii, N Maishi, T Kawamoto, M Morimoto, K Akiyama, Y Yoshioka, T Minami, T Tsumita, MT Alam, T Ochiya, Y Hida, K Hida
    Scientific Reports, 2021-07-05;11(1):13502.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Insomnia symptoms and biomarkers of monocyte activation, systemic inflammation, and coagulation in HIV: Veterans Aging Cohort Study
    Authors: BM Polanka, S Kundu, KA So-Armah, MS Freiberg, SK Gupta, TCB Zapolski, AT Hirsh, RJ Bedimo, MJ Budoff, AA Butt, CH Chang, SS Gottlieb, VC Marconi, JA Womack, JC Stewart
    PLoS ONE, 2021-02-09;16(2):e0246073.
    Species: Human
    Sample Types: Serum
  6. Polarized Cytokine Release Triggered by P2X7 Receptor from Retinal Pigmented Epithelial Cells Dependent on Calcium Influx
    Authors: X Shao, S Guha, W Lu, KE Campagno, JM Beckel, JA Mills, W Yang, CH Mitchell
    Cells, 2020-11-24;9(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Nrf2 Activator PB125� as a Potential Therapeutic Agent Against COVID-19
    Authors: JM McCord, BM Hybertson, A Cota-Gomez, B Gao
    bioRxiv, 2020-06-10;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. The relationship between interleukin-6 levels and physical performance in mobility-limited older adults with chronic low-grade inflammation: The ENRGISE Pilot study
    Authors: C Custodero, SD Anton, DP Beavers, RT Mankowski, SA Lee, MM McDermott, RA Fielding, AB Newman, RP Tracy, SB Kritchevsk, WT Ambrosius, M Pahor, TM Manini
    Arch Gerontol Geriatr, 2020-05-30;90(0):104131.
    Species: Human
    Sample Types: Plasma
  9. Hydrogen gas inhalation alleviates oxidative stress in patients with post-cardiac arrest syndrome
    Authors: T Tamura, M Suzuki, K Hayashida, Y Kobayashi, J Yoshizawa, T Shibusawa, M Sano, S Hori, J Sasaki
    J Clin Biochem Nutr, 2020-04-03;67(2):214-221.
    Species: Human
    Sample Types: Plasma
  10. Association of circulating miR-155 expression level and inflammatory markers with white coat hypertension
    Authors: YQ Huang, C Huang, B Zhang, YQ Feng
    J Hum Hypertens, 2019-09-03;0(0):.
    Species: Human
    Sample Types: Plasma
  11. Maternal pre-pregnancy weight status modifies the influence of PUFAs and inflammatory biomarkers in breastmilk on infant growth
    Authors: H Nuss, A Altazan, J Zabaleta, M Sothern, L Redman
    PLoS ONE, 2019-05-29;14(5):e0217085.
    Species: Human
    Sample Types: Serum
  12. Effects of high temperature on pandemic and seasonal human influenza viral replication and infection-induced damage in primary human tracheal epithelial cell cultures
    Authors: M Yamaya, H Nishimura, N Lusamba Ka, X Deng, H Momma, Y Shimotai, R Nagatomi
    Heliyon, 2019-02-05;5(2):e01149.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Systemic Inflammation Characterizes Lack of Metabolic Health in Nonobese HIV-Infected Men
    Authors: AN Monczor, X Li, FJ Palella, KM Erlandson, D Wiley, LA Kingsley, WS Post, LP Jacobson, TT Brown, JE Lake
    Mediators Inflamm., 2018-09-25;2018(0):5327361.
    Species: Human
    Sample Types: Serum
  14. MicroRNA-874 inhibits TNF-?-induced remodeling in human fetal airway smooth muscle cells by targeting STAT3
    Authors: M Sun, Y Huang, F Li, H Li, B Zhang, L Jin
    Respir Physiol Neurobiol, 2018-02-12;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Association between Fetal Adipokines and Child Behavioral Problems at Preschool Age: The Hokkaido Study on Environment and Children's Health
    Authors: M Minatoya, S Itoh, A Araki, N Tamura, K Yamazaki, C Miyashita, R Kishi
    Int J Environ Res Public Health, 2018-01-11;15(1):.
    Species: Human
    Sample Types: Whole Blood
  16. The Association of Circulating MiR-29b and Interleukin-6 with Subclinical Atherosclerosis
    Authors: YQ Huang, J Li, JY Chen, YL Zhou, AP Cai, C Huang, YQ Feng
    Cell. Physiol. Biochem., 2017-12-04;44(4):1537-1544.
    Species: Human
    Sample Types: Plasma
  17. Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women
    Authors: A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin
    Oncotarget, 2017-09-15;8(54):92134-92142.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity
    Authors: I Bergström, AK Lundberg, S Jönsson, E Särndahl, J Ernerudh, L Jonasson
    PLoS ONE, 2017-03-22;12(3):e0174177.
    Species: Human
    Sample Types: Plasma
  19. N-Terminal Pro-B-Type Natriuretic Peptide as a Biomarker for Loss of Muscle Mass in Prevalent Hemodialysis Patients
    PLoS ONE, 2016-11-21;11(11):e0166804.
    Species: Human
    Sample Types: Serum
  20. Serum biomarkers of inflammation and muscle strength among women with systemic lupus erythematosus
    Cytokine, 2016-11-16;90(0):109-112.
    Species: Human
    Sample Types: Serum
  21. Relevance of Interleukin-6 and D-Dimer for Serious Non-AIDS Morbidity and Death among HIV-Positive Adults on Suppressive Antiretroviral Therapy
    PLoS ONE, 2016-05-12;11(5):e0155100.
    Species: Human
    Sample Types: Plasma
  22. Widespread activation of immunity and pro-inflammatory programs in peripheral blood leukocytes of HIV-infected patients with impaired lung gas exchange
    Authors: K Crothers, I Petrache, C Wongtrakoo, PJ Lee, LM Schnapp, SA Gharib
    Physiol Rep, 2016-04-01;4(8):.
    Species: Human
    Sample Types: Serum
  23. Factors associated with D-dimer levels in HIV-infected individuals.
    Authors: Borges A, O'Connor J, Phillips A, Baker J, Vjecha M, Losso M, Klinker H, Lopardo G, Williams I, Lundgren J
    PLoS ONE, 2014-03-13;9(3):e90978.
    Species: Human
    Sample Types: Plasma
  24. Effect of renal impairment on cognitive function during a 3-year follow up in elderly patients with type 2 diabetes: Association with microinflammation.
    Authors: Kawamura T, Umemura T, Umegaki H, Imamine R, Kawano N, Tanaka C, Kawai M, Minatoguchi M, Kusama M, Kouchi Y, Watarai A, Kanai A, Nakashima E, Hotta N
    J Diabetes Investig, 2014-02-04;5(5):597-605.
    Species: Human
    Sample Types: Plasma
  25. Natural killer (NK) cell deficit in coronary artery disease: no aberrations in phenotype but sustained reduction of NK cells is associated with low-grade inflammation.
    Authors: Backteman K, Ernerudh J, Jonasson L
    Clin Exp Immunol, 2014-01-01;175(1):104-12.
    Species: Human
    Sample Types: Plasma
  26. A pilot study of telmisartan for visceral adiposity in HIV infection: the metabolic abnormalities, telmisartan, and HIV infection (MATH) trial.
    Authors: Lake J, Tseng C, Currier J
    PLoS ONE, 2013-03-14;8(3):e58135.
    Species: Human
    Sample Types: Serum
  27. Angiotensin converting enzyme inhibitor and HMG-CoA reductase inhibitor as adjunct treatment for persons with HIV infection: a feasibility randomized trial.
    Authors: Baker J, Huppler Hullsiek K, Prosser R, Duprez D, Grimm R, Tracy R, Rhame F, Henry K, Neaton J
    PLoS ONE, 2012-10-17;7(10):e46894.
    Species: Human
    Sample Types: Plasma
  28. Inflammation, coagulation and cardiovascular disease in HIV-infected individuals.
    Authors: Duprez, Daniel A, Neuhaus, Jacqueli, Kuller, Lewis H, Tracy, Russell, Belloso, Waldo, De Wit, Stephane, Drummond, Fraser, Lane, H Cliffo, Ledergerber, Bruno, Lundgren, Jens, Nixon, Daniel, Paton, Nicholas, Prineas, Ronald J, Neaton, James D
    PLoS ONE, 2012-09-10;7(9):e44454.
    Species: Human
    Sample Types: Plasma
  29. Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue.
    Authors: Cifuentes M, Fuentes C, Tobar N, Acevedo I, Villalobos E, Hugo E, Ben-Jonathan N, Reyes M
    Mol Cell Endocrinol, 2012-03-16;361(1):24-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Activation of nuclear factor-kappa B signalling promotes cellular senescence.
    Authors: Rovillain E, Mansfield L, Caetano C, Alvarez-Fernandez M, Caballero OL, Medema RH, Hummerich H, Jat PS
    Oncogene, 2011-01-17;30(20):2356-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Early inflammatory and metabolic changes in association with AGTR1 polymorphisms in prehypertensive subjects.
    Authors: Fung MM, Rao F, Poddar S
    Am. J. Hypertens., 2010-09-23;24(2):225-33.
    Species: Human
    Sample Types: Plasma
  32. Biomarkers of inflammation and development of rheumatoid arthritis in women from two prospective cohort studies.
    Authors: Karlson EW, Chibnik LB, Tworoger SS, Lee IM, Buring JE, Shadick NA, Manson JE, Costenbader KH
    Arthritis Rheum., 2009-03-01;60(3):641-52.
    Species: Human
    Sample Types: Plasma
  33. Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
    Authors: Fichorova RN, Richardson-Harman N, Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Curtis K, Dezzutti CS, Donoval B, Doncel GF, Donaghay M, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pasicznyk JM, Pallansch-Cokonis M, Poli G, Reichelderfer P, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R, Cummins JE
    Anal. Chem., 2008-05-17;80(12):4741-51.
    Species: Human
    Sample Types: Reference Standard
  34. Obesity in pregnancy stimulates macrophage accumulation and inflammation in the placenta.
    Authors: Challier JC, Basu S, Bintein T, Minium J, Hotmire K, Catalano PM, Hauguel-De Mouzon S
    Placenta, 2008-02-11;29(3):274-81.
    Species: Human
    Sample Types: Plasma
  35. Usefulness of C-reactive protein and interleukin-6 as predictors of outcomes in patients with chronic obstructive pulmonary disease receiving pravastatin.
    Authors: Lee TM, Lin MS, Chang NC
    Am. J. Cardiol., 2007-12-26;101(4):530-5.
    Species: Human
    Sample Types: Plasma
  36. Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes.
    Authors: Fritzenwanger M, Meusel K, Foerster M, Kuethe F, Krack A, Figulla HR
    Cytokine, 2007-07-16;38(3):137-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma.
    Authors: Feldmann G, Nischalke HD, Nattermann J, Banas B, Berg T, Teschendorf C, Schmiegel W, Duhrsen U, Halangk J, Iwan A, Sauerbruch T, Caselmann WH, Spengler U
    Clin. Cancer Res., 2006-08-01;12(15):4491-8.
    Species: Human
    Sample Types: Serum
  38. Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome in Nagano, Japan: clinical, radiological, and cytokine studies of 13 patients.
    Authors: Oide T, Ohara S, Oguchi K, Maruyama M, Yazawa M, Inoue K, Sekijima Y, Tokuda T, Ikeda S
    Clin. Exp. Rheumatol., 2004-01-01;22(1):91-8.
    Species: Human
    Sample Types: Serum
  39. Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients.
    Authors: Dahaba AA, Elawady GA, Rehak PH, List WF
    Anaesth Intensive Care, 2002-06-01;30(3):269-74.
    Species: Human
    Sample Types: Plasma

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Human IL-6 QuantiGlo ELISA Kit
By Anonymous on 12/09/2021
Sample Tested: Peripheral blood mononuclear cells (PBMCs)

The differences between tocilizumab versus placebo at 8 wk were IL6 measurements.