Human IL-6 QuantiGlo ELISA Kit

  (24 citations)     
Datasheet / CoA / SDS
Product Details
Citations (24)
Supplemental Products
  • Assay Length
    5.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
  • Sensitivity
    0.35 pg/mL
  • Assay Range
    0.5 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-6
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC193 , QuantiGlo Immunoassay Control Set 732 for Human IL-6 - Please Inquire
Product Summary
The QuantiGlo Human IL-6 Chemiluminescent Immunoassay is a solid phase ELISA that specifically measures human IL-6 in less than 5.5 hours. This kit is designed to measure IL-6 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-6 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6. It has been observed in our laboratories that the measurement of human IL-6 is insensitive to the addition of the recombinant form of the human IL-6 soluble receptor.

Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3 4
n 20 20 20 20 20 20 20 20
Mean 1.21 10.7 118 766 1.56 13.2 142 752
Standard Deviation 0.07 0.32 4.15 37.5 0.15 1.02 8.93 53.7
CV% 5.8 3 3.5 4.9 9.6 7.7 6.3 7.1


The recovery of IL-6 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-113
Citrate Plasma (n=4) 87 82-96
EDTA Plasma (n=4) 89 82-95
Heparin Plasma (n=4) 88 80-97
Serum (n=4) 94 88-106
To assess the linearity of the assay, samples spiked with high concentrations of IL-6 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 IL-6 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
  • Long Name:
    Interleukin 6
  • Entrez Gene IDs:
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
  • Alternate Names:
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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Sample Type
  1. The Association of Circulating MiR-29b and Interleukin-6 with Subclinical Atherosclerosis
    Authors: YQ Huang, J Li, JY Chen, YL Zhou, AP Cai, C Huang, YQ Feng
    Cell. Physiol. Biochem., 2017;44(4):1537-1544.
    Species: Human
    Sample Type: Plasma
  2. Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women
    Authors: A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin
    Oncotarget, 2017;8(54):92134-92142.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity
    Authors: I Bergström, AK Lundberg, S Jönsson, E Särndahl, J Ernerudh, L Jonasson
    PLoS ONE, 2017;12(3):e0174177.
    Species: Human
    Sample Type: Plasma
  4. N-Terminal Pro-B-Type Natriuretic Peptide as a Biomarker for Loss of Muscle Mass in Prevalent Hemodialysis Patients
    PLoS ONE, 2016;11(11):e0166804.
    Species: Human
    Sample Type: Serum
  5. Serum biomarkers of inflammation and muscle strength among women with systemic lupus erythematosus
    Cytokine, 2016;90(0):109-112.
    Species: Human
    Sample Type: Serum
  6. Relevance of Interleukin-6 and D-Dimer for Serious Non-AIDS Morbidity and Death among HIV-Positive Adults on Suppressive Antiretroviral Therapy
    PLoS ONE, 2016;11(5):e0155100.
    Species: Human
    Sample Type: Plasma
  7. Widespread activation of immunity and pro-inflammatory programs in peripheral blood leukocytes of HIV-infected patients with impaired lung gas exchange
    Authors: K Crothers, I Petrache, C Wongtrakoo, PJ Lee, LM Schnapp, SA Gharib
    Physiol Rep, 2016;4(8):.
    Species: Human
    Sample Type: Serum
  8. Effect of renal impairment on cognitive function during a 3-year follow up in elderly patients with type 2 diabetes: Association with microinflammation.
    Authors: Kawamura T, Umemura T, Umegaki H, Imamine R, Kawano N, Tanaka C, Kawai M, Minatoguchi M, Kusama M, Kouchi Y, Watarai A, Kanai A, Nakashima E, Hotta N
    J Diabetes Investig, 2014;5(5):597-605.
    Species: Human
    Sample Type: Plasma
  9. Natural killer (NK) cell deficit in coronary artery disease: no aberrations in phenotype but sustained reduction of NK cells is associated with low-grade inflammation.
    Authors: Backteman K, Ernerudh J, Jonasson L
    Clin Exp Immunol, 2014;175(1):104-12.
    Species: Human
    Sample Type: Plasma
  10. Factors associated with D-dimer levels in HIV-infected individuals.
    Authors: Borges A, O'Connor J, Phillips A, Baker J, Vjecha M, Losso M, Klinker H, Lopardo G, Williams I, Lundgren J
    PLoS ONE, 2014;9(3):e90978.
    Species: Human
    Sample Type: Plasma
  11. A pilot study of telmisartan for visceral adiposity in HIV infection: the metabolic abnormalities, telmisartan, and HIV infection (MATH) trial.
    Authors: Lake J, Tseng C, Currier J
    PLoS ONE, 2013;8(3):e58135.
    Species: Human
    Sample Type: Serum
  12. Angiotensin converting enzyme inhibitor and HMG-CoA reductase inhibitor as adjunct treatment for persons with HIV infection: a feasibility randomized trial.
    Authors: Baker J, Huppler Hullsiek K, Prosser R, Duprez D, Grimm R, Tracy R, Rhame F, Henry K, Neaton J
    PLoS ONE, 2012;7(10):e46894.
    Species: Human
    Sample Type: Plasma
  13. Inflammation, coagulation and cardiovascular disease in HIV-infected individuals.
    Authors: Duprez, Daniel A, Neuhaus, Jacqueli, Kuller, Lewis H, Tracy, Russell, Belloso, Waldo, De Wit, Stephane, Drummond, Fraser, Lane, H Cliffo, Ledergerber, Bruno, Lundgren, Jens, Nixon, Daniel, Paton, Nicholas, Prineas, Ronald J, Neaton, James D
    PLoS ONE, 2012;7(9):e44454.
    Species: Human
    Sample Type: Plasma
  14. Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue.
    Authors: Cifuentes M, Fuentes C, Tobar N, Acevedo I, Villalobos E, Hugo E, Ben-Jonathan N, Reyes M
    Mol Cell Endocrinol, 2012;361(1):24-30.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Early inflammatory and metabolic changes in association with AGTR1 polymorphisms in prehypertensive subjects.
    Authors: Fung MM, Rao F, Poddar S
    Am. J. Hypertens., 2011;24(2):225-33.
    Species: Human
    Sample Type: Plasma
  16. Activation of nuclear factor-kappa B signalling promotes cellular senescence.
    Authors: Rovillain E, Mansfield L, Caetano C, Alvarez-Fernandez M, Caballero OL, Medema RH, Hummerich H, Jat PS
    Oncogene, 2011;30(20):2356-66.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. Biomarkers of inflammation and development of rheumatoid arthritis in women from two prospective cohort studies.
    Authors: Karlson EW, Chibnik LB, Tworoger SS, Lee IM, Buring JE, Shadick NA, Manson JE, Costenbader KH
    Arthritis Rheum., 2009;60(3):641-52.
    Species: Human
    Sample Type: Plasma
  18. Obesity in pregnancy stimulates macrophage accumulation and inflammation in the placenta.
    Authors: Challier JC, Basu S, Bintein T, Minium J, Hotmire K, Catalano PM, Hauguel-De Mouzon S
    Placenta, 2008;29(3):274-81.
    Species: Human
    Sample Type: Plasma
  19. Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
    Authors: Fichorova RN, Richardson-Harman N, Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Curtis K, Dezzutti CS, Donoval B, Doncel GF, Donaghay M, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pasicznyk JM, Pallansch-Cokonis M, Poli G, Reichelderfer P, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R, Cummins JE
    Anal. Chem., 2008;80(12):4741-51.
    Species: Human
    Sample Type: Reference Standard
  20. Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes.
    Authors: Fritzenwanger M, Meusel K, Foerster M, Kuethe F, Krack A, Figulla HR
    Cytokine, 2007;38(3):137-44.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. Usefulness of C-reactive protein and interleukin-6 as predictors of outcomes in patients with chronic obstructive pulmonary disease receiving pravastatin.
    Authors: Lee TM, Lin MS, Chang NC
    Am. J. Cardiol., 2007;101(4):530-5.
    Species: Human
    Sample Type: Plasma
  22. Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma.
    Authors: Feldmann G, Nischalke HD, Nattermann J, Banas B, Berg T, Teschendorf C, Schmiegel W, Duhrsen U, Halangk J, Iwan A, Sauerbruch T, Caselmann WH, Spengler U
    Clin. Cancer Res., 2006;12(15):4491-8.
    Species: Human
    Sample Type: Serum
  23. Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome in Nagano, Japan: clinical, radiological, and cytokine studies of 13 patients.
    Authors: Oide T, Ohara S, Oguchi K, Maruyama M, Yazawa M, Inoue K, Sekijima Y, Tokuda T, Ikeda S
    Clin. Exp. Rheumatol., 2004;22(1):91-8.
    Species: Human
    Sample Type: Serum
  24. Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients.
    Authors: Dahaba AA, Elawady GA, Rehak PH, List WF
    Anaesth Intensive Care, 2002;30(3):269-74.
    Species: Human
    Sample Type: Plasma
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QuantiGlo Immunoassay Control Set 732 for Human IL-6

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