Human IL-8/CXCL8 Quantikine HS ELISA Kit

(8 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    0.4 pg/mL
  • Assay Range
    1.0 - 64 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-8
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC41, Quantikine Immunoassay Control Group 10 - Please Inquire
Product Summary
The Quantikine HS Human IL-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-8 levels in serum and plasma. It contains E. coli-expressed recombinant human IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-8 accurately. Results obtained using natural human IL-8 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean5.518.737.1519.439.2
Standard Deviation0.30.72.70.41.63.7
CV%5.53.77.388.29.4

Recovery

The recovery of IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 95 86-102
Heparin Plasma (n=4) 99 91-107
Serum (n=4) 99 85-110
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-8/CXCL8 Quantikine HS ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-8/CXCL8
Interleukin-8 (IL-8), also known as CXCL8, is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. IL-8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. IL-8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. IL-8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents IL-8 interaction with CXCR1.
    • Long Name
      Interleukin 8
    • Entrez Gene IDs
      3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline);
    • Alternate Names
      3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 6 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Do not wash the plate.

    13. 200 µL Amplifier Solution
    14.   Add 200 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.

    15. 50 µL Stop Solution
    16. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    8 Citations: Showing 1 - 8
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    Species
    Sample Type
    1. Exogenous growth factors bFGF, EGF and HGF do not influence viability and phenotype of V600EBRAF melanoma cells and their response to vemurafenib and trametinib in vitro
      Authors: I Zalesna, M Osrodek, ML Hartman, M Rozanski, M Sztiller-S, K Niewinna, D Nejc, M Czyz
      PLoS ONE, 2017;12(8):e0183498.
      Species: Human
      Sample Type: Whole Cells
    2. Mindfulness and its efficacy for psychological and biological responses in women with breast cancer
      Authors: E Kenne Sare, LB Mårtensson, BA Andersson, P Karlsson, I Bergh
      Cancer Med, 2017;0(0):.
      Species: Human
      Sample Type: Serum
    3. Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
      Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
      Cell Death Dis, 2017;8(8):e3017.
      Species: Human
      Sample Type: Serum
    4. Endothelial activation by platelets from sickle cell anemia patients.
      Authors: Proenca-Ferreira R, Brugnerotto A, Garrido V, Dominical V, Vital D, Ribeiro M, dos Santos M, Traina F, Olalla-Saad S, Costa F, Conran N
      PLoS ONE, 2014;9(2):e89012.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study.
      Authors: Pisetsky D, Trace S, Brownley K, Hamer R, Zucker N, Roux-Lombard P, Dayer J, Bulik C
      Cytokine, 2014;69(1):110-5.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for "inflamm-aging"
      Authors: Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, Benatti S, Gibellini L, Cotichini R, Stazi M, Trenti T, Franceschi C, Cossarizza A
      Eur J Immunol, 2014;44(5):1552-62.
      Species: Human
      Sample Type: Plasma
    7. Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease.
      Authors: Singh R, Mackay A, Patel A, Garcha D, Kowlessar B, Brill S, Donnelly L, Barnes P, Donaldson G, Wedzicha J
      Respir Res, 2014;15(0):114.
      Species: Human
      Sample Type: Sputum
    8. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
      Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
      PLoS ONE, 2013;8(12):e83042.
      Species: Human
      Sample Type: Plasma
    ELISA Controls
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    Quantikine Immunoassay Control Group 10

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