Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 560201
View all Integrin alpha 9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Integrin alpha 9
Val89-Leu169
Accession # Q13797

Specificity

Detects human Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin alpha 1, 2, 2b, 3, 4, 5, 6, 6 (x1), 8, 10, 11, D, E, L, M, V, X, recombinant mouse Integrin alpha 7 (x2) or 9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha 9 Antibody

Detection of Human Integrin a9 antibody by Western Blot.

Detection of Human Integrin alpha 9 by Western Blot.

Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Integrin a9 Monoclonal Antibody (Catalog # MAB4574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Integrin alpha 9 by Western Blot

Detection of Human Integrin alpha 9 by Western Blot

Expression of VEGF-D-binding receptors by 468LN cells:(A) Expression of VEGF-R2 and R3 mRNA examined with semi quantitative RT-PCR, and (B) alpha 9 integrin mRNA measured with qRT-PCR. (C) Confirmation of VEGF-R2, VEGF-R3, and alpha 9 integrin expression at protein levels by western blot. MDA-MB-231 and MCF7-COX-2 cells were used as positive controls primarily for VEGF-R2 and VEGF-R3 expression. Differentially high alpha 9 integrin expression by 468 LN cells was evident. Bars represent mean (n = 4) ± SE, *,P<0.05. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0035094), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Integrin  alpha 9 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Tera2 human embryonic lung carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB4574 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 9

Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 Integrin. alpha 9 beta 1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse Integrin  alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg 566‑Val 567). The ECD of mouse Integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human Integrin alpha 9, respectively.

Alternate Names

ITGA4L, ITGA9, RLC-alpha

Entrez Gene IDs

3680 (Human); 104099 (Mouse); 685004 (Rat)

Gene Symbol

ITGA9

UniProt

Q13797

Additional Integrin alpha 9 Products

Product Documents for Human Integrin  alpha 9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  alpha 9 Antibody

For research use only

Citations for Human Integrin  alpha 9 Antibody

Customer Reviews for Human Integrin  alpha 9 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Huh-7 human hepatoma cell line
    Species: Mouse
    Verified Customer | Posted 04/30/2018

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