Detects human Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin alpha 1, 2, 2b, 3, 4, 5, 6, 6 (x1), 8, 10, 11, D, E, L, M, V, X, recombinant mouse Integrin alpha 7 (x2) or 9 is observed.
Monoclonal Mouse IgG1 Clone # 560201
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Integrin alpha 9 Val89-Leu169 Accession # Q13797
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Integrin alpha 9 by Western Blot.
Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Integrin alpha 9 Monoclonal Antibody (Catalog # MAB4574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin alpha 9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 9
Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 Integrin. alpha 9 beta 1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse Integrin alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg 566‑Val 567). The ECD of mouse Integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human Integrin alpha 9, respectively.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you
include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image.
Limited to verified customers in USA, Canada and Europe.
Read what people are saying who have used Human Integrin alpha 9 Antibody.
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Western Blot.