Human Integrin  alpha 9 Antibody

Catalog # Availability Size / Price Qty
MAB4574
MAB4574-SP

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Detection of Human Integrin  alpha 9 by Western Blot.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human Integrin  alpha 9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin alpha 1, 2, 2b, 3, 4, 5, 6, 6 (x1), 8, 10, 11, D, E, L, M, V, X, recombinant mouse Integrin alpha 7 (x2) or 9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 560201
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Integrin alpha 9
Val89-Leu169
Accession # Q13797
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Integrin a9 antibody by Western Blot. View Larger

Detection of Human Integrin alpha 9 by Western Blot. Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Integrin a9 Monoclonal Antibody (Catalog # MAB4574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human Integrin alpha 9 by Western Blot View Larger

Detection of Human Integrin alpha 9 by Western Blot Expression of VEGF-D-binding receptors by 468LN cells:(A) Expression of VEGF-R2 and R3 mRNA examined with semi quantitative RT-PCR, and (B) alpha 9 integrin mRNA measured with qRT-PCR. (C) Confirmation of VEGF-R2, VEGF-R3, and alpha 9 integrin expression at protein levels by western blot. MDA-MB-231 and MCF7-COX-2 cells were used as positive controls primarily for VEGF-R2 and VEGF-R3 expression. Differentially high alpha 9 integrin expression by 468 LN cells was evident. Bars represent mean (n = 4) ± SE, *,P<0.05. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0035094), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 9

Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 Integrin. alpha 9 beta 1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse Integrin  alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg 566‑Val 567). The ECD of mouse Integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human Integrin alpha 9, respectively.

Entrez Gene IDs
3680 (Human); 104099 (Mouse); 685004 (Rat)
Alternate Names
ALPHA-RLC; Integrin alpha 9; integrin alpha-9; Integrin alpha-RLC; integrin, alpha 9; ITGA4L; ITGA9; RLC; RLC-alpha

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Citations for Human Integrin  alpha 9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. An EPHB4-RASA1 signaling complex inhibits shear stress-induced Ras-MAPK activation in lymphatic endothelial cells to promote the development of lymphatic vessel valves
    Authors: Chen, D;Wiggins, D;Sevick, EM;Davis, MJ;King, PD;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Angiogenesis in the Outer Membrane of Chronic Subdural Hematomas through Thrombin-Cleaved Osteopontin and the Integrin ?9 and Integrin ?1 Signaling Pathways
    Authors: Osuka, K;Ohmichi, Y;Ohmichi, M;Honma, S;Suzuki, C;Aoyama, M;Iwami, K;Watanabe, Y;Miyachi, S;
    Biomedicines
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Western Blot, IHC
  3. Pharmacological inhibition of FOXO1 promotes lymphatic valve growth in a congenital lymphedema mouse model
    Authors: Ogunsina O, Banerjee R, Knauer LA, Yang Y.
    Frontiers in Cell and Developmental Biology
  4. Piezo1 incorporates mechanical force signals to genetic program that governs lymphatic valve development and maintenance
    Authors: D Choi, E Park, E Jung, B Cha, S Lee, J Yu, PM Kim, S Lee, YJ Hong, CJ Koh, CW Cho, Y Wu, NL Jeon, AK Wong, L Shin, SR Kumar, I Bermejo-Mo, RS Srinivasan, IT Cho, YK Hong
    JCI Insight, 2019-03-07;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  5. Co-expression of alpha9beta1 integrin and VEGF-D confers lymphatic metastatic ability to a human breast cancer cell line MDA-MB-468LN.
    Authors: Majumder M, Tutunea-Fatan E, Xin X, Rodriguez-Torres M, Torres-Garcia J, Wiebe R, Timoshenko AV, Bhattacharjee RN, Chambers AF, Lala PK
    PLoS ONE, 2012-04-24;7(4):e35094.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human Integrin  alpha 9 Antibody

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Human Integrin  alpha 9 Antibody
By Anonymous on 04/30/2018
Application: WB Sample Tested: Huh-7 human hepatoma cell line Species: Mouse