Human Intelectin-1/Omentin Antibody

Catalog # Availability Size / Price Qty
AF4254
AF4254-SP

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Intelectin‑1/Omentin in Mouse Intestine.
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Product Details
Citations (8)
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Human Intelectin-1/Omentin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Intelectin-1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Intelectin-1/Omentin
Thr19-Ser298
Accession # Q8WWA0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Intelectin‑1/Omentin
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Intelectin‑1/Omentin antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

Intelectin‑1/Omentin in Mouse Intestine. Intelectin-1/Omentin was detected in immersion fixed frozen sections of mouse intestine using 10 µg/mL Sheep Anti-Human Intelectin-1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunocytochemistry Intelectin-1/Omentin antibody in U937 Human Cell Line by Immunocytochemistry (ICC). View Larger

Intelectin‑1/Omentin in U937 Human Cell Line. Intelectin-1/Omentin was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-Human Intelectin-1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Intelectin‑1/Omentin in ileum ALI. Intelectin‑1/Omentin was detected in immersion fixed sections of ileum ALI using Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to mucus granules in goblet cells. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry/ Immunofluorescence Detection of Human Intelectin-1/Omentin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Intelectin-1/Omentin by Immunocytochemistry/Immunofluorescence SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). Points coloured red indicate genes significant at 5% permutation test. Selected highly significant genes are labelled. b Bar chart showing the percentage of cells in the ‘goblet-type’ cell cluster (n = 98) expressing MUC2, ITLN1 or SPINK4 alone or in different combinations (thresholds set at the tenth centile to include 90% of cells in which at least one transcript was detected from each gene). c Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal colon from a resection specimen (blue stain is DAPI). Scale bar, 100 µm. d Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal oesophageal epithelium obtained by endoscopic biopsy (blue stain is DAPI). OSGs encroaching on the surface epithelium are shown in the enlarged images on the right. Scale bars are 200 µm and 50 µm in enlarged images. e Triple immunofluorescence staining images of KRT14 (white), KRT7 (green) and MUC2 (red) in an OSG beneath normal squamous epithelium from an endoscopic biopsy of normal squamous epithelium from a patient with BO biopsy (blue stain is DAPI). Scale bar 50 µm. f Representative immunofluorescence staining of Barrett’s EMR specimen containing intestinal metaplasia but no dysplasia for MUC2 (red), ITLN1 (white) and SPINK4 (green); nuclei (DAPI) in blue. Scale bars are 400 µm and 100 µm in enlarged images Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30323168), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Intelectin-1/Omentin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Intelectin-1/Omentin by Immunocytochemistry/Immunofluorescence SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). Points coloured red indicate genes significant at 5% permutation test. Selected highly significant genes are labelled. b Bar chart showing the percentage of cells in the ‘goblet-type’ cell cluster (n = 98) expressing MUC2, ITLN1 or SPINK4 alone or in different combinations (thresholds set at the tenth centile to include 90% of cells in which at least one transcript was detected from each gene). c Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal colon from a resection specimen (blue stain is DAPI). Scale bar, 100 µm. d Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal oesophageal epithelium obtained by endoscopic biopsy (blue stain is DAPI). OSGs encroaching on the surface epithelium are shown in the enlarged images on the right. Scale bars are 200 µm and 50 µm in enlarged images. e Triple immunofluorescence staining images of KRT14 (white), KRT7 (green) and MUC2 (red) in an OSG beneath normal squamous epithelium from an endoscopic biopsy of normal squamous epithelium from a patient with BO biopsy (blue stain is DAPI). Scale bar 50 µm. f Representative immunofluorescence staining of Barrett’s EMR specimen containing intestinal metaplasia but no dysplasia for MUC2 (red), ITLN1 (white) and SPINK4 (green); nuclei (DAPI) in blue. Scale bars are 400 µm and 100 µm in enlarged images Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30323168), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Intelectin-1/Omentin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Intelectin-1/Omentin by Immunocytochemistry/Immunofluorescence SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). Points coloured red indicate genes significant at 5% permutation test. Selected highly significant genes are labelled. b Bar chart showing the percentage of cells in the ‘goblet-type’ cell cluster (n = 98) expressing MUC2, ITLN1 or SPINK4 alone or in different combinations (thresholds set at the tenth centile to include 90% of cells in which at least one transcript was detected from each gene). c Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal colon from a resection specimen (blue stain is DAPI). Scale bar, 100 µm. d Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal oesophageal epithelium obtained by endoscopic biopsy (blue stain is DAPI). OSGs encroaching on the surface epithelium are shown in the enlarged images on the right. Scale bars are 200 µm and 50 µm in enlarged images. e Triple immunofluorescence staining images of KRT14 (white), KRT7 (green) and MUC2 (red) in an OSG beneath normal squamous epithelium from an endoscopic biopsy of normal squamous epithelium from a patient with BO biopsy (blue stain is DAPI). Scale bar 50 µm. f Representative immunofluorescence staining of Barrett’s EMR specimen containing intestinal metaplasia but no dysplasia for MUC2 (red), ITLN1 (white) and SPINK4 (green); nuclei (DAPI) in blue. Scale bars are 400 µm and 100 µm in enlarged images Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30323168), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-1/Omentin

Intelectin-1 (intestinal lectin; also ITLN-1 and omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.

Entrez Gene IDs
55600 (Human); 16429 (Mouse)
Alternate Names
Endothelial lectin HL-1; FLJ20022; Galactofuranose-binding lectin; hIntL; HL-1; intelectin 1 (galactofuranose binding); Intelectin1; Intelectin-1; INTLHL1; ITLN1; Itlna; ITLNITLN-1; LFR; LFRIntestinal lactoferrin receptor; Omentin; Omentin-1

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Citations for Human Intelectin-1/Omentin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Intelectin-1 binds and alters the localization of the mucus barrier-modifying bacterium Akkermansia muciniphila
    Authors: Juan D. Matute, Jinzhi Duan, Magdalena B. Flak, Paul Griebel, Jose A. Tascon-Arcila, Shauni Doms et al.
    J Exp Med
  2. Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: a possible involvement in the fattening and the onset of puberty
    Authors: A Barbe, P Kurowska, E Mlyczy?ska, C Ramé, C Staub, E Venturi, Y Billon, A Rak, J Dupont
    Gen. Comp. Endocrinol., 2020-08-19;0(0):113584.
    Species: Porcine
    Sample Types: Plasma, Tissue Lysate
    Applications: Western Blot
  3. Functional characterization of a subtilisin-like serine protease from Vibrio cholerae
    Authors: M Howell, DG Dumitrescu, LR Blankenshi, D Herkert, SK Hatzios
    J. Biol. Chem., 2019-05-10;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  4. Single cell RNA-seq reveals profound transcriptional similarity between Barrett’s oesophagus and oesophageal submucosal glands
    Authors: Richard Peter Owen, Michael Joseph White, David Tyler Severson, Barbara Braden, Adam Bailey, Robert Goldin et al.
    Nature Communications
  5. Chemoproteomic profiling of host and pathogen enzymes active in cholera
    Authors: Stavroula K. Hatzios, Sören Abel, Julianne Martell, Troy Hubbard, Jumpei Sasabe, Diana Munera et al.
    Nature Chemical Biology
  6. Recognition of microbial glycans by human intelectin-1
    Authors: Darryl A. Wesener, Kittikhun Wangkanont, Ryan McBride, Xuezheng Song, Matthew B. Kraft, Heather L. Hodges et al.
    Nature Structural & Molecular Biology
  7. Cardioprotective properties of omentin-1 in type 2 diabetes: evidence from clinical and in vitro studies.
    Authors: Greulich, Sabrina, Chen, Weena J, Maxhera, Bujar, Rijzewijk, Luuk J, van der Meer, Rutger W, Jonker, Jacqueli, Mueller, Heidi, de Wiza, Daniella, Floerke, Ralf-Rue, Smiris, Konstant, Lamb, Hildo J, de Roos, Albert, Bax, Jeroen J, Romijn, Johannes, Smit, Jan W A, Akhyari, Payam, Lichtenberg, Artur, Eckel, Juergen, Diamant, Michaela, Ouwens, D Margri
    PLoS ONE, 2013-03-29;8(3):e59697.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation.
    Authors: Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P
    J. Biol. Chem., 2011-09-12;286(44):38231-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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