Human IRF8 Antibody

Catalog # Availability Size / Price Qty
Detection of Human IRF8 by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products

Human IRF8 Antibody Summary

Species Reactivity
Detects human IRF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human IRF1, 2, 3, 4, 5, 6, 7, and 9.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human IRF8
Accession # Q02556
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IRF8 antibody by Western Blot. View Larger

Detection of Human IRF8 by Western Blot. Western blot shows lysates of human liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IRF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5117) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF8 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry IRF8 antibody in Human Lymphoma by Immunohistochemistry (IHC-P). View Larger

IRF8 in Human Lymphoma. IRF8 was detected in immersion fixed paraffin-embedded sections of human lymphoma using 5 µg/mL Sheep Anti-Human IRF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5117) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF8

IRF8 (interferon regulatory factor 8; also ICSBP) is a 50 kDa member of the IRF family of proteins. It is expressed in myeloid, dendritic and B cells and interacts with PU.1 to regulate the expression of TLR4, IL-18, IL-12p35, and PTPN13, a Fas-associated phosphatase that blocks Fas-induced apoptosis. Human IRF8 is 426 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 9-110) and at least one regulatory phosphorylation site at Tyr95. Over aa 255-426, human IRF8 shares 90% and 88% aa sequence identity with canine and mouse IRF8, respectively.

Long Name
Interferon Regulatory Factor 8
Entrez Gene IDs
3394 (Human); 15900 (Mouse); 292060 (Rat)
Alternate Names
ICSBP1; ICSBPH-ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; interferon regulatory factor 8; IRF8; IRF-8ICSBP1

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Citation for Human IRF8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Wilms' tumor gene 1 protein represses the expression of the tumor suppressor interferon regulatory factor 8 in human hematopoietic progenitors and in leukemic cells.
    Authors: Vidovic K, Svensson E, Nilsson B, Thuresson B, Olofsson T, Lennartsson A, Gullberg U
    Leukemia, 2010-03-18;24(5):992-1000.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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