Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all IRF8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRF8
Ala255-Val426
Accession # Q02556

Specificity

Detects human IRF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human IRF1, 2, 3, 4, 5, 6, 7, and 9.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human IRF8 Antibody

Detection of Human IRF8 antibody by Western Blot.

Detection of Human IRF8 by Western Blot.

Western blot shows lysates of human liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IRF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5117) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF8 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

IRF8 antibody in Human Lymphoma by Immunohistochemistry (IHC-P).

IRF8 in Human Lymphoma.

IRF8 was detected in immersion fixed paraffin-embedded sections of human lymphoma using 5 µg/mL Sheep Anti-Human IRF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5117) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human IRF8 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymphoma

Western Blot

1 µg/mL
Sample: Human liver and kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRF8

IRF8 (interferon regulatory factor 8; also ICSBP) is a 50 kDa member of the IRF family of proteins. It is expressed in myeloid, dendritic and B cells and interacts with PU.1 to regulate the expression of TLR4, IL-18, IL-12p35, and PTPN13, a Fas-associated phosphatase that blocks Fas-induced apoptosis. Human IRF8 is 426 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 9-110) and at least one regulatory phosphorylation site at Tyr95. Over aa 255-426, human IRF8 shares 90% and 88% aa sequence identity with canine and mouse IRF8, respectively.

Long Name

Interferon Regulatory Factor 8

Alternate Names

ICSBP1

Entrez Gene IDs

3394 (Human); 15900 (Mouse); 292060 (Rat)

Gene Symbol

IRF8

UniProt

Q02556

Additional IRF8 Products

Product Documents for Human IRF8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRF8 Antibody

For research use only

Citations for Human IRF8 Antibody

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Protocols

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