Human IRS2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human IRS2 by Western Blot.
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Product Details
Supplemental Products

Human IRS2 Antibody Summary

Species Reactivity
Detects human IRS2 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human IRS2
Accession # Q9Y4H2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IRS2 antibody by Western Blot. View Larger

Detection of Human IRS2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human IRS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6347) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRS2 at approximately 170-190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRS2

IRS2 (insulin receptor substrate 2) is a 180-190 kDa substrate intermediate that lies between various cytokine receptors and downstream signaling molecules. It is the primary IRS protein found in hematopoietic cells. Depending upon the receptor, IRS2 undergoes phosphorylation, either via Tyk2 following type I IFN binding, or via JAK1 and 3 following IL-4, -7 and -15 binding. This event allows for its subsequent association with PI-3 kinase. Human IRS2 is 1338 amino acids (aa) in length with a predicted MW of 138 kDa. It contains one PH (pleckstrin homology) domain (aa 16-144), followed by a PTB (phosphotyrosine-binding)domain (aa 194-298) and seven YxxM (Tyr/x/x/Met) motifs (aa 540-1075). IRS2 is phosphorylated on multiple Ser and Tyr residues. There are two potential splice variants. One shows a 16 aa substitution for the C-terminal Glu, while another shows an 11 aa substitution for aa 1314-1338. Over aa 1035-1136, human IRS2 shares 86% aa identity with mouse IRS2. 

Long Name
Insulin Receptor Substrate 2
Entrez Gene IDs
8660 (Human); 384783 (Mouse); 29376 (Rat)
Alternate Names
insulin receptor substrate 2; IRS2; IRS-2

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