Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 323803
View all Kallikrein 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 1
Ile25-Ser262
Accession # NP_002248

Specificity

Detects human Kallikrein 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) KLK-3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, -14, rhHGFA, rhFactorVII, rhFactorXI, rhThrombin or rhUPA-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Kallikrein 1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 1 (Catalog # 2337-SE)

Western Blot

1 µg/mL
Sample: Recombinant Human Kallikrein 1 (Catalog # 2337-SE) under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB2337 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kallikrein 1

Kallikrein 1 (KLK1), also known as tissue kallikrein, is a member of human tissue kallikrein family (1). The best known physiological function of KLK1 is the cleavage of kininogen to release the vasoactive kinin peptide (bradykinin or lysyl-bradykinin), which regulates vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation (2). In addition, KLK1 may play a role in angiogensis and tumorigenesis (2). Human KLK1 precursor contains a signal peptide (residues1 to 18), a short pro peptide (residues 19 to 24) and a mature chain (residues 25 to 262). The purified rhKLK1 contains the mature chain and the propeptide from human KLK5. After being activated by thermolysin, rhKLK1 is active against a fluorogenic peptide substrate described above.

References

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Chao, J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1577.

Alternate Names

KLK1

Entrez Gene IDs

3816 (Human); 16612 (Mouse)

Gene Symbol

KLK1

UniProt

NP_002248

Additional Kallikrein 1 Products

Product Documents for Human Kallikrein 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 1 Antibody

For research use only

Customer Reviews for Human Kallikrein 1 Antibody (1)

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 21211543
    Species: Human
    Verified Customer | Posted 02/19/2015

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