Human Kallikrein 12 Antibody

Catalog # Availability Size / Price Qty
AF3095
AF3095-SP
Detection of Kallikrein 12 in Human Duodenum.
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Product Details
Citations (1)
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Human Kallikrein 12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Kallikrein 8, rhKallikrein 10, rhKallikrein 11, and rhKallikrein 15 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 12
Ala18-Asn248
Accession # Q9UKR0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Kallikrein 12 (Catalog # 3095-SE)
Immunohistochemistry
3-15 µg/mL
Immersion fixed paraffin-embedded  sections of Human Duodenum
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 12 (Catalog # 3095-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Kallikrein 12 in Human Duodenum. Kallikrein 12 was detected in immersion fixed paraffin-embedded sections of Human Duodenum using Sheep Anti-Human Kallikrein 12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3095) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells in intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 12

Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family. It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The KLK12 produced by the R&D Systems corresponds to the full-length classical form, also known as isoform 2 (1). The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat. The recombinant human KLK12 can be autoactivated under the conditions described in the Activity Assay Protocol. The active enzyme has the N-terminal sequence of I22FNGTECGRNS.

References
  1. Yousef, G.M. et al. (2000) Genomics. 69:331.
  2. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Entrez Gene IDs
43849 (Human)
Alternate Names
DKFZp686H1078; EC 3.4.21; EC 3.4.21.-; Kallikrein 12; Kallikrein-like protein 5; kallikrein-related peptidase 12; KLK12; KLK-L5; KLKL5kallikrein-12; KLK-L5MGC42603

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Citation for Human Kallikrein 12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix
    Authors: T Kryza, C Parent, J Pardessus, A Petit, J Burlaud-Ga, P Reverdiau, S Iochmann, V Labas, Y Courty, N Heuzé-Vour
    Sci Rep, 2018-04-20;8(1):6331.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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