Human Kallikrein 2 Antibody

  (1 citations)     
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Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human Kallikrein 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kallikrein 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or B1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 426723
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Kallikrein 2
    Ile25-Pro261
    Accession # P20151
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Kallikrein 2 (Catalog # 4104-SE).
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Kallikrein 2 (Catalog # 4104-SE), see our available Western blot detection antibodies
  • Neutralization
    Measured by its ability to neutralize Recombinant Human Kallikrein 2 (2 µg/mL, Catalog # 4104-SE) cleavage of the fluorogenic peptide substrate PFR-AMC (0.1 mM). The Neutralization Dose (ND50) is typically 3.4 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 2
Kallikrein 2 (KLK2) is a secreted serine protease that is highly expressed in the human prostate gland (1). It is also known as the prostate-specific glandular kallikrein. The enzyme is highly specific for cleavage after arginine residues. KLK2 is able to activate the urokinase-type plasminogen activator (2). KLK2 is inhibited by serpins such as protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1 (3‑5). KLK2 is structurally related to KLK3, the prostate-specific antigen (PSA). Like PSA, KLK2 is considered to be a biomarker for prostate cancer (6).
  • References:
    1. Chapelaine, P. et al. (1988) FEBS Lett. 236:205.
    2. Frenette, G. et al. (1997) Int. J. Cancer 71:897.
    3. Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
    4. Grauer, L.S. et al. (1998) J. Androl. 19:407.
    5. Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
    6. Sardana, G. et al. (2008) Clin. Chem. 54:1951.
  • Entrez Gene IDs:
    3817 (Human); 24841 (Rat)
  • Alternate Names:
    EC 3.4.21; EC 3.4.21.35; glandular kallikrein 2; Glandular kallikrein-1; hGK-1; hK2; Kallikrein 2; kallikrein 2, prostatic; kallikrein-2; kallikrein-related peptidase 2; Klk1c2; KLK2; KLK2A2; MGC12201; Tissue kallikrein-2; Ton
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Direct B cell stimulation by dendritic cells in a mouse model of lupus.
    Authors: Wan S, Zhou Z, Duan B, Morel L
    Arthritis Rheum., 2008;8(0):1741-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut

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