Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Kallikrein 6/Neurosin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 6
Glu17-Lys244
Accession # Q92876

Specificity

Detects human Kallikrein 6 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Kallikrein 3, rhKallikrein 5, rhKallikrein 11, rhKallikrein 13, rhKallikrein 14, and rhKallikrein 15 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Kallikrein 6/Neurosin Antibody

Kallikrein 6/Neurosin antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Kallikrein 6/Neurosin in Human Breast Cancer Tissue.

Kallikrein 6/Neurosin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human Kallikrein 6/Neurosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2008) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Kallikrein 6/Neurosin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 6/Neurosin (Catalog # 5164-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Kallikrein 6/Neurosin (Catalog # 5164-SE)

Reviewed Applications

Read 2 reviews rated 4 using AF2008 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kallikrein 6/Neurosin

Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members (1, 2). The genes of this family aligned in tandem on chromosome 19q13.4 represent the largest contiguous group of proteases within the human genome. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung (2, 3). Human Kallikrein 6 (hKLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes (1-3). Known protein substrates of hKLK6 are myelin basic protein, the precursor of the A beta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The level of hKLK6 is reduced in brain extracts of Alzheimer patients and increased in serum of patients with ovarian cancer.

References

  1. Emami, N. and E.P. Diamandis (2007) Clin. Chim. Acta 381:78.
  2. Borgoño, C.A. and E.P. Diamandis (2004) Nat. Rev. Cancer 4:876.
  3. Paliouras, M. et al. (2007) Cancer Lett. 249:61.

Alternate Names

KLK6, Neurosin, Protease M, PRSS18, PRSS9, SP59, Zyme

Entrez Gene IDs

5653 (Human)

Gene Symbol

KLK6

UniProt

Q92876

Additional Kallikrein 6/Neurosin Products

Product Documents for Human Kallikrein 6/Neurosin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 6/Neurosin Antibody

For research use only

Citations for Human Kallikrein 6/Neurosin Antibody

Customer Reviews for Human Kallikrein 6/Neurosin Antibody (2)

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  • Human Kallikrein 6/Neurosin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell lysate from colon cancer cell line
    Species: Human
    Verified Customer | Posted 04/26/2016
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 20846516
    Species: Human
    Verified Customer | Posted 01/06/2015

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Protocols

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