Human Kallikrein 6/Neurosin Antibody

Catalog # Availability Size / Price Qty
AF2008
AF2008-SP
Human Kallikrein 6/Neurosin Antibody in Immunohistochemistry
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human Kallikrein 6/Neurosin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 6 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Kallikrein 3, rhKallikrein 5, rhKallikrein 11, rhKallikrein 13, rhKallikrein 14, and rhKallikrein 15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 6
Glu17-Lys244
Accession # Q92876
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Kallikrein 6/Neurosin (Catalog # 5164-SE)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 6/Neurosin (Catalog # 5164-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Kallikrein 6/Neurosin in Human Breast Cancer Tissue. View Larger

Kallikrein 6/Neurosin in Human Breast Cancer Tissue. Kallikrein 6/Neurosin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human Kallikrein 6/Neurosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2008) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 6/Neurosin

Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members (1, 2). The genes of this family aligned in tandem on chromosome 19q13.4 represent the largest contiguous group of proteases within the human genome. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung (2, 3). Human Kallikrein 6 (hKLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes (1-3). Known protein substrates of hKLK6 are myelin basic protein, the precursor of the A beta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The level of hKLK6 is reduced in brain extracts of Alzheimer patients and increased in serum of patients with ovarian cancer.

References
  1. Emami, N. and E.P. Diamandis (2007) Clin. Chim. Acta 381:78.
  2. Borgoño, C.A. and E.P. Diamandis (2004) Nat. Rev. Cancer 4:876.
  3. Paliouras, M. et al. (2007) Cancer Lett. 249:61.
Entrez Gene IDs
5653 (Human)
Alternate Names
Bssp; EC 3.4.21; EC 3.4.21.-; hK6; kallikrein 6 (neurosin, zyme); Kallikrein 6; kallikrein-6; kallikrein-related peptidase 6; KLK6; Klk7; Neurosin; Protease M; protease, serine, 18; PRSS18; PRSS9; PRSS9MGC9355; Serine protease 18; Serine protease 9; SP59; Zyme

Product Datasheets

Citations for Human Kallikrein 6/Neurosin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Expression of Kallikrein-Related Peptidase 6 in Primary Mucosal Malignant Melanoma of the Head and Neck
    Authors: Jochen Hess
    Head Neck Pathol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Kallikrein-related peptidase 6 regulates epithelial-to-mesenchymal transition and serves as prognostic biomarker for head and neck squamous cell carcinoma patients.
    Authors: Schrader C, Kolb M, Zaoui K, Flechtenmacher C, Grabe N, Weber K, Hielscher T, Plinkert P, Hess J
    Mol Cancer, 2015;14(0):107.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study.
    Authors: Munoz-Repeto I, Garcia M, Kamieniak M, Ramon Y Cajal T, Domingo S, Cazorla A, Garcia Donas J, Hernando Polo S, Garcia Sagredo J, Hernandez E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benitez J
    Histol Histopathol, 2013;28(1):133-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  4. Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma.
    Authors: Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD
    Biol. Chem., 2007;388(11):1235-42.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Kallikrein 6/Neurosin Antibody

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Human Kallikrein 6/Neurosin Antibody
By Anonymous on 04/26/2016
Application: WB Sample Tested: Cell lysate from colon cancer cell line Species: Human