hh Human KCC2/SLC12A5 Antibody MAB8369: R&D Systems

Human KCC2/SLC12A5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SLC12A5 in ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 905705
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human SLC12A5
    Accession # Q9H2X9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human KCC2/SLC12A5 by Western Blot. Western blot shows lysates of human brain stem tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for KCC2/SLC12A5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of KCC2/SLC12A5 in HEK293 Human Cell Line Transfected with Human KCC2/SLC12A5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human KCC2/SLC12A5 and eGFP was stained with either (A) Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KCC2/SLC12A5

Solute Carrier, family 12 (potassium/chloride transporter), member 5 (SLC12A5) encodes KCC2, a multi-pass co-transporter for K+ and Cl-.  KCC2/SLC12A5 is expressed on neurons, and is dysregulated in schizophrenia, spasticity, and affective disorders.

  • Long Name:
    K-Cl Cotransporter 2
  • Entrez Gene IDs:
    57468 (Human); 57138 (Mouse); 171373 (Rat)
  • Alternate Names:
    Electroneutral potassium-chloride cotransporter 2; hKCC2; KCC2; KCC2K-Cl cotransporter 2; KIAA1176erythroid K-Cl cotransporter 2; Neuronal K-Cl cotransporter; SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; solute carrier family 12 member 5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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