Human KCC2/SLC12A5 Antibody

Catalog # Availability Size / Price Qty
MAB8369
MAB8369-SP
Detection of Human KCC2/SLC12A5 by Western Blot.
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Human KCC2/SLC12A5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SLC12A5 in ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 905705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human SLC12A5
Accession # Q9H2X9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human KCC2/SLC12A5 antibody by Western Blot. View Larger

Detection of Human KCC2/SLC12A5 by Western Blot. Western blot shows lysates of human brain stem tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for KCC2/SLC12A5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of KCC2/SLC12A5 antibody in HEK293 Human Cell Line Transfected with Human KCC2/SLC12A5 and eGFP antibody by Flow Cytometry. View Larger

Detection of KCC2/SLC12A5 in HEK293 Human Cell Line Transfected with Human KCC2/SLC12A5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human KCC2/SLC12A5 and eGFP was stained with either (A) Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KCC2/SLC12A5

Solute Carrier, family 12 (potassium/chloride transporter), member 5 (SLC12A5) encodes KCC2, a multi-pass co-transporter for K+ and Cl-.  KCC2/SLC12A5 is expressed on neurons, and is dysregulated in schizophrenia, spasticity, and affective disorders.

Long Name
K-Cl Cotransporter 2
Entrez Gene IDs
57468 (Human); 57138 (Mouse); 171373 (Rat)
Alternate Names
Electroneutral potassium-chloride cotransporter 2; hKCC2; KCC2; KCC2K-Cl cotransporter 2; KIAA1176erythroid K-Cl cotransporter 2; Neuronal K-Cl cotransporter; SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; solute carrier family 12 member 5

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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