Human KIR3DL2/CD158k Antibody (539304)

R&D Systems | Catalog # MAB2878

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry, Depletion

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 539304
View all KIR3DL2/CD158k Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human KIR3DL2/CD158k
Accession # P43630

Specificity

Detects human KIR3DL2/CD158k in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human KIR3DL2/CD158k Antibody (539304)

Detection of KIR3DL2/CD158k antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of KIR3DL2/CD158k in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human KIR3DL2/CD158k Monoclonal Antibody (Catalog # MAB2878) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of KIR3DL2/CD158k by Flow Cytometry

Detection of KIR3DL2/CD158k by Flow Cytometry

The expression of KIR3DL2 in HCC and its clinical significance in determining prognosis of HCC patients(A) The expression profile of KIR3DL2 in tumor and adjacent non-tumor tissues across various cancers. ∗:p < 0.05; ∗∗:p < 0.01; ∗∗∗: p < 0.001.(B) The different expression of KIR3DL2 in tumor and adjacent non-tumor tissues from Geo database.(C) The landscape of clinical feature of HCC patients related to KIR3DL2 in TCGA and ICGC database.(D) Kaplan-Meier analysis of KIR3DL2 for survival of HCC patients from TCGA database. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2589004224018625), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of KIR3DL2/CD158k by Flow Cytometry

Detection of KIR3DL2/CD158k by Flow Cytometry

Flow cytometry detection of KIR3DL2 expression on NK cells in peripheral blood, adjacent tissues, and tumor tissues of HCC patients(A) Flow cytometry gating strategy.(B) Expression of KIR3DL2 on NK cells in peripheral blood of healthy individuals and HCC patients, as well as in adjacent and tumor tissues from HCC patients.(C) The correlation between KIR3DL2 expression on NK cells in peripheral blood and in adjacent and tumor tissues. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2589004224018625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human KIR3DL2/CD158k Antibody (539304)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood lymphocytes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR3DL2/CD158k

KIR3DL2 (3DL2, p140, CD158k) is a type I transmembrane protein of the p70 family of killer cell Ig-like receptors (KIR). KIR are expressed on CD56dim NK cells and T cell subsets where they participate in identifying normal and abnormal cells and regulating effector functions of the innate immune system (1-4). KIR are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD) and whether they have long or short (L, S) cytoplasmic tails. Like other inhibitory KIR, KIR3DL2 has two ITIM domains within its long tail (3). KIR3DL2 is diverse, with twelve alleles identified and as many as five single amino acid polymorphisms found in a single individual (4-6). Unlike most other KIR, gene transcripts of KIR3DL2 are expressed by all individuals (4). KIR3DL2 is present on the cell surface as a disulfide-linked homodimer of 70 kDa, 434 aa subunits (4). KIR3DL2 has shown peptide-specific binding to some HLA-A antigens, including A3 and A11 (4, 7, 8).  It also binds the abnormally folded HLA-B27 homodimer found in spondylarthritides, but not the normal heterodimer of HLA-B27 with beta 2‑microglobulin (9, 10). NK and CD4+ T cells from patients with spondylarthritides show increased KIR3DL2+ expression and may play a role in disease pathology (10). KIR3DL2 is also a marker for atypical mononuclear (Sezary) cells in the blood of patients with Sezary syndrome, an erythrodermic form of cutaneous T cell lymphoma (11). Human KIR3DL2 ECD shows 88-92% aa identity to KIR3DL2 of other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly-49 proteins are functional orthologs (3). KIR are highly related. The closest relative, KIR3DL1 shows 86% aa identity with KIR3DL2 within the ECD.

References

  1. Colonna, M. and J. Samaridis (1995) Science 268:405.
  2. Pende, D. et al. (1996) J. Exp. Med. 184:505.
  3. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  4. Uhrberg, M. et al. (1997) Immunity 7:753.
  5. Meenaugh, A. et al. (2004) Tiss. Antigens 64:226.
  6. Kwon, D. et al. (2000) Mol. Cells 10:54.
  7. Dohring, C. et al. (1996) J. Immunol. 156:3098.
  8. Hansasuta, P. et al. (2004) Eur. J. Immunol. 34:1673.
  9. Kollnberger, S. et al. (2002) Arthritis Rheum. 46:2972.
  10. Chan, A. T. et al. (2005) Arthritis Rheum. 52:3586.
  11. Poszepczynska-Guigne, E. et al. (2004) J. Invest. Dermatol. 122:820.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 2

Alternate Names

CD158k, CL-5, NKAT4A, NKAT4B

Entrez Gene IDs

3812 (Human); 245615 (Mouse)

Gene Symbol

KIR3DL2

UniProt

P43630

Additional KIR3DL2/CD158k Products

Product Documents for Human KIR3DL2/CD158k Antibody (539304)

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Product Specific Notices for Human KIR3DL2/CD158k Antibody (539304)

For research use only

Citations for Human KIR3DL2/CD158k Antibody (539304)

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