Human Laminin alpha 4 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Laminin alpha 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse Laminin alpha 4 is observed.
• Source
  Polyclonal Sheep IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Chinese hamster ovary cell line CHO-derived recombinant human Laminin alpha 4 Gln826-Ala1816, predicted Accession # EAW48267
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Endotoxin Level
  <0.10 EU per 1 μg of the antibody by the LAL method.
• Label
  Unconjugated

• Western Blot
  2 µg/mL
  See below
• Immunocytochemistry
  5-15 µg/mL
  See below
• Neutralization
  Measured by its ability to neutralize Laminin  alpha 4-induced adhesion in the HT1080 human fibrosarcoma cell line. The Neutralization Dose (ND₅₀) is typically 1.5-7.5 µg/mL in the presence of 5 µg/mL Recombinant Human Laminin  alpha 4.

Western Blot click image to view larger	Detection of Human Laminin  alpha 4 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Laminin  alpha 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Laminin  alpha 4 at approximately 200‑220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry click image to view larger	Laminin  alpha 4 in T98G Human Cell Line. Laminin  alpha 4 was detected in immersion fixed T98G human glioblastoma cell line, induced (upper panel) or not (lower panel) with StemXVivo EMT Inducing Media Supplement (Catalog # CCM017), using Sheep Anti-Human Laminin  alpha 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7340) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Neutralization click image to view larger	Adhesion Induced by Laminin  alpha 4 and Neutralization by Human Laminin  alpha 4 Antibody. Recombinant Human Laminin alpha 4 (Catalog # 7340-A4) supports adhesion in the HT1080 human fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Laminin  alpha 4 (5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human Laminin  alpha 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7340). The ND50 is typically 1.5-7.5 µg/mL.

• Reconstitution
  Sterile PBS to a final concentration of 0.2 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

• Entrez Gene IDs:
  3910 (Human); 16775 (Mouse); 309816 (Rat)

• Alternate Names:
  DKFZp686D23145; LAMA3LAMA4*-1; LAMA4; laminin alpha 4 chain; Laminin alpha 4; laminin subunit alpha-4; laminin, alpha 4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha

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