Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 877005
View all LC3A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LC3A
Accession # Q9H492

Specificity

Detects human LC3A in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human LC3A Antibody

Detection of Human, Mouse, and Rat LC3A antibody by Western Blot.

Detection of Human, Mouse, and Rat LC3A by Western Blot.

Western blot shows lysates of human brain tissue, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 50 µM Chloroquine for 18 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human LC3A Antibody (Catalog # MAB8558) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for LC3A at approximately 14 and 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LC3A antibody in Human Brain Cortex Tissue by Immunohistochemistry (IHC-P).

LC3A in Human Brain Cortex Tissue.

LC3A was detected in immersion fixed paraffin-embedded sections of human brain cortex tissue using Rat Anti-Human LC3A Monoclonal Antibody (Catalog # MAB8558) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
LC3A antibody in Human Brain by Immunohistochemistry (IHC-P).

LC3A in Human Brain.

LC3A was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Rat Anti-Human LC3A Monoclonal Antibody (Catalog # MAB8558) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human LC3A Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex tissue

Western Blot

2 µg/mL
Sample: Human brain tissue (untreated), NIH‑3T3 mouse embryonic fibroblast cell line and PC‑12 rat adrenal pheochromocytoma cell line treated with Chloroquine

Reviewed Applications

Read 2 reviews rated 5 using MAB8558 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LC3A

Human Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of Autophagy-related 8 (Atg8) proteins (1). The LC3 subfamily also includes LC3B andLC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins (3). However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation (4). Recruitment of LC3 to the phagophore may promote membrane elongation (4,5). It may also be involved in cargo recruitment to autophagosomes (1). LC3 is often used as a marker of autophagy. 

References

  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. He, H. et al. (2003) J. Biol. Chem. 278:29278.
  3. Kuznetsov, S.A. & V.I. Gelfand (1987) FEBS Let. 212:145.
  4. Weidberg, H. et al. (2011) Ann Rev. Biochem. 80:125.
  5. Weidberg, H. et al. (2010) EMBO J. 29:1792. 

Long Name

Microtubule-associated Protein 1 Light Chain 3 alpha

Alternate Names

Apg8, APG8a, Apg8p3, ATG8E, LC3, MAP1ALC3, MAP1LC3A, MLP3A

Entrez Gene IDs

84557 (Human); 66734 (Mouse); 362245 (Rat); 852200 (Yeast)

Gene Symbol

MAP1LC3A

UniProt

Q9H492

Additional LC3A Products

Product Documents for Human LC3A Antibody

Certificate of Analysis

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Product Specific Notices for Human LC3A Antibody

For research use only

Citations for Human LC3A Antibody

Customer Reviews for Human LC3A Antibody (2)

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  • Human LC3A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: gastric cancer
    Species: Human
    Verified Customer | Posted 10/29/2021
    Human LC3A Antibody MAB8558
  • Human LC3A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 08/30/2017

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Protocols

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