Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 600007
View all LILRA2/CD85h/ILT1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRA2/CD85h/ILT1
Pro17-Ser437
Accession # Q8N149

Specificity

Detects human LILRA2/CD85h/ILT1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT2, 3, 4, 5, rhLIR6, or rhLIR8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LILRA2/CD85h/ILT1 Antibody

Detection of LILRA2/CD85h/ILT1 antibody in Human PBMC antibody by Flow Cytometry.

Detection of LILRA2/CD85h/ILT1 in Human PBMC by Flow Cytometry.

Human peripheral blood monocytes were stained with Human LILRA2/CD85h/ILT1 Monoclonal Antibody (Catalog # MAB6364, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Applications for Human LILRA2/CD85h/ILT1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes

Reviewed Applications

Read 1 review rated 4 using MAB6364 in the following applications:

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA2/CD85h/ILT1

Immune-like transcript 1 (ILT1), also known as leukocyte immunoglobulin-like receptor 7 (LIR-7), leukocyte immunoglobulin-like receptor subfamily A member 2 (LILRA2), and CD85H, is a 51 kDa (unglycosylated) type I transmembrane glycoprotein and member of the leukocyte immunoglobulin-like receptor family. Human ILT1 is synthesized as a 483 amino acid (aa) precursor that contains a 23 aa signal sequence, a 426 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic region. The ECD contains four Ig-like C2-type domains and seven potential sites for N-linked glycosylation. A splice variant produces a second isoform that has a one aa substitution for aa 419-436 in the longer form. There are no murine orthologs for human ILT1. ILT1 may act as a receptor for class I MHC antigens.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 2

Alternate Names

CD85h, ILT1, LIR7

Entrez Gene IDs

11027 (Human)

Gene Symbol

LILRA2

UniProt

Q8N149 (Human)

Additional LILRA2/CD85h/ILT1 Products

Product Documents for Human LILRA2/CD85h/ILT1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LILRA2/CD85h/ILT1 Antibody

For research use only

Customer Reviews for Human LILRA2/CD85h/ILT1 Antibody (1)

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Customer Images

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  • Human LILRA2/CD85h/ILT1 Antibody
    Name: Jan Martinek
    Application: Immunofluorescence-Frozen
    Sample Tested: human melanoma
    Species: Human
    Verified Customer | Posted 11/15/2018
    Human melanoma stained for ILT1 (red) counterstained with DAPI (blue)
    Acetone fixation
    Human LILRA2/CD85h/ILT1 Antibody MAB6364

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