Human LINGO-2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LINGO-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LINGO-1 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 382007
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LINGO-2
    Cys28-Leu542
    Accession # Q7L985
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human LINGO‑2 under non-reducing conditions only
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed A549 human lung carcinoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LINGO‑2 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Human LINGO‑2 Monoclonal Antibody (Catalog # MAB36791, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
Detection of LINGO‑2 in HEK293 Human Cell Line Transfected with Human LINGO-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line either (A) transfected with human LINGO-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LINGO‑2 Monoclonal Antibody (Catalog # MAB36791) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LINGO-2

Human LINGO-2 (LRR and Ig domain-containing, Nogo Receptor-interacting protein 2; also known as Leucine-rich repeat neuronal 6C (LRRN6C) or LERN3), type I transmembrane protein in the neuronal leucine-rich repeat family. These proteins have a signal peptide, 12 extracellular leucine-rich repeats flanked by N-terminal and C-terminal cysteine-rich sequences, an immunoglobulin-like domain, a transmembrane domain and a short cytoplasmic tail.  An alternate start site may exist at Met148 of the precursor. Human LINGO-2 is a highly conserved, 606 amino acid protein that shares 99% and 98% aa sequence identity with canine and mouse LINGO-2, respectively. LINGO-2 presumably functions outside the CNS with little involvement by p75/NgR1.

  • Long Name:
    Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 2
  • Entrez Gene IDs:
    158038 (Human); 242384 (Mouse); 313156 (Rat)
  • Alternate Names:
    LERN3; LERN3FLJ31810; leucine rich repeat and Ig domain containing 2; leucine-rich repeat and immunoglobulin-like domain-containing nogoreceptor-interacting protein 2; Leucine-rich repeat neuronal protein 3; Leucine-rich repeat neuronal protein 6C; LINGO2; LINGO-2; LRRN6C; LRRN6Cleucine rich repeat neuronal 6C
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