Detection of LINGO‑2 in A549 Human Cell Line by Flow Cytometry.
A549 human lung carcinoma cell line was stained with Human LINGO‑2 Monoclonal Antibody (Catalog # MAB36791, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
Detection of LINGO‑2 in HEK293 Human Cell Line Transfected with Human LINGO-2 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line either (A) transfected with human LINGO-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LINGO‑2 Monoclonal Antibody (Catalog # MAB36791) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human LINGO-2 (LRR and Ig domain-containing, Nogo Receptor-interacting protein 2; also known as Leucine-rich repeat neuronal 6C (LRRN6C) or LERN3), type I transmembrane protein in the neuronal leucine-rich repeat family.
These proteins have a signal peptide, 12 extracellular leucine-rich
repeats flanked by N-terminal and C-terminal cysteine-rich sequences, an
immunoglobulin-like domain, a transmembrane domain and a short
cytoplasmic tail. An alternate start site may exist at Met148 of the precursor. Human LINGO-2 is a highly conserved, 606 amino acid protein that shares 99% and 98% aa sequence identity with canine and mouse LINGO-2, respectively. LINGO-2 presumably functions outside the CNS with little involvement by p75/NgR1.
Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 2
Entrez Gene IDs:
158038 (Human); 242384 (Mouse); 313156 (Rat)
LERN3; LERN3FLJ31810; leucine rich repeat and Ig domain containing 2; leucine-rich repeat and immunoglobulin-like domain-containing nogoreceptor-interacting protein 2; Leucine-rich repeat neuronal protein 3; Leucine-rich repeat neuronal protein 6C; LINGO2; LINGO-2; LRRN6C; LRRN6Cleucine rich repeat neuronal 6C
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