Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 220308
View all Lipocalin-2/NGAL Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lipocalin-2
Gln21-Gly198
Accession # P80188

Specificity

Detects human Lipocalin-2 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) Lipocalin-1, rhMMP-9, rmLipocalin-2, or rrLipocalin-2.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Lipocalin‑2/NGAL Antibody

Human Lipocalin‑2/NGAL ELISA Standard Curve.

Recombinant Human Lipocalin‑2/NGAL protein was serially diluted 2-fold and captured by Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB17571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB17573) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Lipocalin‑2/NGAL Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB17571).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lipocalin-2/NGAL

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin‑2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin‑2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3 - 5). Studies indicate that Lipocalin‑2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin‑2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

References

  1. Flower, D.R. et al. (1994) FEBS Lett. 354:7.
  2. Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293: 829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Entrez Gene IDs

3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)

Gene Symbol

LCN2

UniProt

P80188

Additional Lipocalin-2/NGAL Products

Product Documents for Human Lipocalin‑2/NGAL Antibody

Certificate of Analysis

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Product Specific Notices for Human Lipocalin‑2/NGAL Antibody

For research use only

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