Detection of Human LRG1 by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRG1 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LRG1 in Human Brain.
LRG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LRG-1 (Leucine-Rich alpha-2-Glycoprotein 1; also LRHG [in rodent]) is a 45-55 kDa, monomeric, variably glycosylated, secreted protein member of the leucine-rich repeat family of molecules. It should not be confused with either LRIG-1/ Leucine-Rich repeats and Ig-like domains-1, or LRG1/LIM-RhoGap protein 1. LRG-1 has a limited expression pattern, being associated with neutrophils, neutrophil precursors (myelocytes; metamyelocytes), astrocytes, HEV (in mice), hepatocytes and select tumor cells. LRG-1 is reported to bind multiple molecules, including TGF-beta, extracellular cytochrome C, and fibronectin plus collagen IV of the ECM. Functions associated with this binding include granulocyte maturation, protection from apoptosis, and cell migration. Mature human LRG-1 is 312 amino acids (aa) in length (aa 35-347). Depending upon the source, LRG-1 has been described as having either thirteen 24 aa leucine-rich repeats (LRRs), or eight LRRs spanning aa 93-282 coupled to a C-terminal LRR. Variable O- and N-based glycosylation accounts for the variablility in MW. Mature human LRG-1 shares 66% aa sequence identity with mouse LRG-1.
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