Human LRP-1 Cluster II Antibody

Catalog # Availability Size / Price Qty
MAB2368-100
MAB2368-SP
Detection of Human LRP‑1 Cluster II by Western Blot.
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Human LRP-1 Cluster II Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRP-1 Cluster II in ELISA and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 1034124
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived human LRP-1 Cluster II
Gln4449-Ala4544
Accession # Q07954
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
U-87 MG human glioblastoma/astrocytoma cell line and U-118-MG human glioblastoma/astrocytoma cell line
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human LRP‑1 Cluster II by Western Blot. Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP‑1 Cluster II Monoclonal Antibody (Catalog # MAB2368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for LRP‑1 Cluster II at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry View Larger

LRP‑1 Cluster II in Human Brain (Cortex). LRP‑1 Cluster II was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human LRP‑1 Cluster II Monoclonal Antibody (Catalog # MAB2368) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-1 Cluster II

LRP-1 (low-density lipoprotein receptor-related protein 1) is a large type I transmembrane protein belonging to the LDL receptor superfamily. The extracellular domain of LRP-1 is organized in four clusters of ligand-binding repeats that recognizes at least 30 different ligands. The amino acid sequence of human and mouse LRP-1 cluster II share 100% identity. LRP-1 is an endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. It plays a crucial role in early embryonic development.

Long Name
LDL Receptor-related Protein 1, Cluster II
Entrez Gene IDs
4035 (Human); 16971 (Mouse); 299858 (Rat)
Alternate Names
A2MR; A2MR;APOER;APR;CD91;IGFBP3R;IGFBP-3R;IGFBP3R1;KPA;LRP;LRP1A;Prolow-density lipoprotein receptor-related protein 1;TGFBR5; Alpha-2-Macroglobulin Receptor; APOER; APR; CD91; LRP1 Cluster II; LRP-1 Cluster II; LRP1; TGFBR5

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