Human LRP-1 Cluster III Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LRP-1 Cluster III in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human LRP-1 Cluster II is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived human LRP-1 Cluster III
    Ser2522-Ile2941
    Accession # Q07954
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
LRP‑1 Cluster III in MG‑63 Human Cell Line. LRP‑1 Cluster III was detected in immersion fixed MG‑63 human osteosarcoma cell line using Sheep Anti-Human LRP‑1 Cluster III Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRP-1 Cluster III

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I membrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and complexes of serine proteases with their inhibitors (1-4). LRP-1 also associates directly or through intracellular scaffold proteins with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta, NMDA receptor subunits, TGF-beta receptors, Frizzled‑1, various integrins, and the prion protein PrPC (1, 5‑10). Human LRP-1 is an N‑glycosylated and sialylated molecule that is cleaved in the Golgi to produce an 85 kDa transmembrane  beta chain and a 515 kDa  alpha chain that associates noncovalently with the  beta chain but does not itself cross the membrane (11, 12). The alpha chain of LRP-1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF‑like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). LRP-1 Cluster III (aa 2522‑2941) contains ten LDLR-A cysteine-rich domains (14). Within this region, human LRP-1 shares 97% aa sequence identity with mouse and rat LRP-1. A soluble form of LRP-1 is shed into the serum and cerebrospinal fluid and retains ligand binding properties (15, 16). LRP-1 Cluster III contains binding sites for LRPAP/RAP (14).

  • References:
    1. Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
    2. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
    3. Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
    4. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
    5. Muratoglu, S.C. et al. (2010) J. Biol. Chem. 258:14308.
    6. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
    7. Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
    8. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
    9. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
    10. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
    11. Herz, J. et al. (1990) EMBO J. 9:1769.
    12. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
    13. Herz, J. et al. (1988) EMBO J. 7:4119.
    14. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
    15. Liu, Q. et al. (2009) Mol. Neurodegener. 4:17.
    16. Gorovoy, M. et al. (2010) J. Leukoc. Biol. Jul 7 epub.
  • Long Name:
    LDL Receptor-related Protein 1, Cluster III
  • Entrez Gene IDs:
    4035 (Human); 16971 (Mouse); 299858 (Rat)
  • Alternate Names:
    LRP1 Cluster III; LRP-1 Cluster III
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