LRP‑1 Cluster III in MG‑63 Human Cell Line.
LRP‑1 Cluster III was detected in immersion fixed MG‑63 human osteosarcoma cell line using Sheep Anti-Human LRP‑1 Cluster III Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRP-1 Cluster III
LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I membrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and complexes of serine proteases with their inhibitors (1-4). LRP-1 also associates directly or through intracellular scaffold proteins with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta, NMDA receptor subunits, TGF-beta receptors, Frizzled‑1, various integrins, and the prion protein PrPC (1, 5‑10). Human LRP-1 is an N‑glycosylated and sialylated molecule that is cleaved in the Golgi to produce an 85 kDa transmembrane beta chain and a 515 kDa alpha chain that associates noncovalently with the beta chain but does not itself cross the membrane (11, 12). The alpha chain of LRP-1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF‑like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). LRP-1 Cluster III (aa 2522‑2941) contains ten LDLR-A cysteine-rich domains (14). Within this region, human LRP-1 shares 97% aa sequence identity with mouse and rat LRP-1. A soluble form of LRP-1 is shed into the serum and cerebrospinal fluid and retains ligand binding properties (15, 16). LRP-1 Cluster III contains binding sites for LRPAP/RAP (14).
Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
Muratoglu, S.C. et al. (2010) J. Biol. Chem. 258:14308.
Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
Herz, J. et al. (1990) EMBO J. 9:1769.
Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
Herz, J. et al. (1988) EMBO J. 7:4119.
Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
Liu, Q. et al. (2009) Mol. Neurodegener. 4:17.
Gorovoy, M. et al. (2010) J. Leukoc. Biol. Jul 7 epub.
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