Western blot shows lysates of Jurkat human acute T cell leukemia cell line and PANC‑1 human pancreatic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRP-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRP-11 at approximately 60 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LRP-11 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRP-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8355) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LRP11 (Low-density lipoprotein receptor-related protein 11) is a 500 amino acid single-pass type I membrane glycoprotein that exists as 2 alternatively spliced isoforms with predicted molecular weights of 53 and 25 kDa, observed 60 and 35 kDa respectively. LRP11 is a novel member of the LDLR family of proteins. Over aa 38-450, human LRP11 shares 67% aa identity with mouse LRP11.
LDL Receptor-related Protein 11
Entrez Gene IDs:
84918 (Human); 237253 (Mouse); 292462 (Rat)
bA350J20.3; FLJ14735; low density lipoprotein receptor-related protein 11; low-density lipoprotein receptor-related protein 11; LRP11; LRP-11; MANSC3; MGC39092
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