* Provided that the recommended microplates, buffers, diluents, substrates and solutions
are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human LRRC32 / GARP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
TGF-beta binding proteins 1-4/Latent TGF-beta binding proteins 1-4 (LTBPs 1-4) are members of the LTBP/fibrillin family of proteins. They are characterized by the presence of multiple EGF-like repeats and a series of cysteine-rich repeats. Extracellularly, LTBPs form part of a large inactive TGF-beta complex where they serve to anchor a mature, dimeric TGF-beta/TGF-beta propeptide complex to the ECM.
Entrez Gene IDs:
2615 (Human); 434215 (Mouse);
Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32
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