Human Lumican DuoSet ELISA
Human Lumican DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Lumican
Lumican belongs to the Small Leucine-rich Proteoglycan (SLRP) family/Class II subfamily of proteins. Expressed in the extracellular matrix, cornea, and other tissues, Lumican can exist as a glycoprotein lacking keratan sulfate or as a proteoglycan depending on its location.
Citations for Human Lumican DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Prediction of emergency cerclage outcomes in women with cervical insufficiency: The role of inflammatory, angiogenic, and extracellular matrix-related proteins in amniotic fluid
Authors: KN Lee, KH Park, YM Kim, I Cho, TE Kim
PLoS ONE, 2022;17(5):e0268291.
Species: Human
Sample Types: Amniotic Fluid
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Association of SLRPs with carotid artery atherosclerosis in essential hypertensive patients
Authors: Y Yang, QH Wu, Y Li, PJ Gao
J Hum Hypertens, 2018;0(0):.
Species: Human
Sample Types: Serum
FAQs
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Reviews for Human Lumican DuoSet ELISA
Average Rating: 3.8 (Based on 4 Reviews)
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std curve is good but samples show same Optical density readings for all dilutions used (from 1:2 to 1:500) so I could not establish a suitable use for this kit.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
