Human Lumican DuoSet ELISA

Catalog # Availability Size / Price Qty
DY2846-05
Ancillary Products Available
Human Lumican ELISA Standard Curve
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human Lumican DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human Lumican ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lumican

Lumican belongs to the Small Leucine-rich Proteoglycan (SLRP) family/Class II subfamily of proteins. Expressed in the extracellular matrix, cornea, and other tissues, Lumican can exist as a glycoprotein lacking keratan sulfate or as a proteoglycan depending on its location.

Entrez Gene IDs:
4060 (Human); 17022 (Mouse)
Alternate Names:
KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican

Citation for Human Lumican DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Association of SLRPs with carotid artery atherosclerosis in essential hypertensive patients
    Authors: Y Yang, QH Wu, Y Li, PJ Gao
    J Hum Hypertens, 2018;0(0):.
    Species: Human
    Sample Types: Serum

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Human Lumican DuoSet ELISA

Average Rating: 4 (Based on 1 Review)

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Human Lumican DuoSet ELISA
By Anonymous on 09/18/2018
Sample Tested: Serum and Plasma