Human Lumican DuoSet ELISA

R&D Systems | Catalog # DY2846-05

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

125-8000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Lumican DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Lumican ELISA Kits »

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Lumican DuoSet ELISA

Human Lumican ELISA Standard Curve

Human Lumican ELISA Standard Curve

Kit Contents for Human Lumican DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lumican

Lumican belongs to the Small Leucine-rich Proteoglycan (SLRP) family/Class II subfamily of proteins. Expressed in the extracellular matrix, cornea, and other tissues, Lumican can exist as a glycoprotein lacking keratan sulfate or as a proteoglycan depending on its location.

Alternate Names

LDC, LUM, SLRR2D

Entrez Gene IDs

4060 (Human); 17022 (Mouse)

Gene Symbol

LUM

Additional Lumican Products

Product Documents for Human Lumican DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Lumican DuoSet ELISA

For research use only

Related Research Areas

Proteoglycans

Citations for Human Lumican DuoSet ELISA

Customer Reviews for Human Lumican DuoSet ELISA (4)

3.8 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
0%
2 Stars
25%
1 Stars
0%

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Showing  1 - 4 of 4 reviews Showing All
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  • Human Lumican DuoSet ELISA
    Name: Nadezhda Glezeva
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 11/22/2021
    std curve is good but samples show same Optical density readings for all dilutions used (from 1:2 to 1:500) so I could not establish a suitable use for this kit.
    Human Lumican DuoSet ELISA DY2846-05
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Human Lumican DuoSet ELISA
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 04/11/2019
  • Human Lumican DuoSet ELISA
    Name: Julie Van
    Sample Tested: Urine
    Verified Customer | Posted 03/07/2019
    Human Lumican DuoSet ELISA DY2846-05
  • Human Lumican DuoSet ELISA
    Name: Christian Cassel
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 09/18/2018

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