Human MD-2 Antibody

Catalog # Availability Size / Price Qty
MAB1787
MAB1787-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human MD-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MD-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human MD-1.
Source
Monoclonal Mouse IgG1 Clone # 288307
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MD-2
Glu17-Asn160
Accession # Q9Y6Y9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MD‑2 (Catalog # 1787-MD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MD2

MD-2, also known as lymphocyte antigen 96 and ESOP-1, is a secreted glycoprotein that associates with the extracellular domain of TLR4. MD-2 is required for LPS binding and subsequent TLR4 signaling.

Long Name
Myeloid Differentiation Protein 2/Lymphocyte Antigen 96
Entrez Gene IDs
23643 (Human); 17087 (Mouse); 102118398 (Cynomolgus Monkey)
Alternate Names
ESOP1; ESOP-1; LY96; ly-96; lymphocyte antigen 96; MD2; MD-2; myeloid differentiation protein-2; Protein MD-2

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Citations for Human MD-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Soluble CD83 Inhibits T Cell Activation by Binding to the TLR4/MD-2 Complex on CD14(+) Monocytes
    Authors: JM Horvatinov, EW Grogan, M Norris, A Steinkasse, H Lemos, AL Mellor, IY Tcherepano, CA Nicolette, MA DeBenedett
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression.
    Authors: Macagno A, Molteni M, Rinaldi A
    J. Exp. Med., 2006;203(6):1481-92.
    Species: Human
    Sample Types: Buffer
    Applications: ELISA Development

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MD-2 Antibody

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Human MD-2 Antibody
By Anonymous on 03/29/2021
Application: ELISA Sample Tested: wheat extracts Species: wheat

we use this antibody to see interactions with TLR4/MD2 complex and MD2 recombinant protein. Works well at a minimum of 1:10 folds in direct ELISA.