Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 288307
View all MD2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MD-2
Glu17-Asn160
Accession # Q9Y6Y9

Specificity

Detects human MD-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human MD-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human MD‑2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human MD‑2 (Catalog # 1787-MD)

Reviewed Applications

Read 1 review rated 4 using MAB1787 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MD2

MD-2, also known as lymphocyte antigen 96 and ESOP-1, is a secreted glycoprotein that associates with the extracellular domain of TLR4. MD-2 is required for LPS binding and subsequent TLR4 signaling.

Long Name

Myeloid Differentiation Protein 2/Lymphocyte Antigen 96

Alternate Names

ESOP-1, LY96, MD-2

Entrez Gene IDs

23643 (Human); 17087 (Mouse); 102118398 (Cynomolgus Monkey)

Gene Symbol

LY96

UniProt

Q9Y6Y9

Additional MD2 Products

Product Documents for Human MD‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MD‑2 Antibody

For research use only

Related Research Areas

Monocyte Markers
Toll-Like Receptors

Citations for Human MD‑2 Antibody

Customer Reviews for Human MD‑2 Antibody (1)

4 out of 5
1 Customer Rating
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  • Human MD-2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: wheat extracts
    Species: wheat
    Verified Customer | Posted 03/29/2021
    we use this antibody to see interactions with TLR4/MD2 complex and MD2 recombinant protein. Works well at a minimum of 1:10 folds in direct ELISA.
    Human MD‑2 Antibody MAB1787

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