Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MEGF9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MEGF9
Ala36-Asn514
Accession # Q9H1U4

Specificity

Detects human MEGF9 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human MEGF9 Antibody

Detection of Human MEGF9 antibody by Western Blot.

Detection of Human MEGF9 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MEGF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MEGF9 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MEGF9 antibody in Human Brain by Immunohistochemistry (IHC-P).

MEGF9 in Human Brain.

MEGF9 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human MEGF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7768) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MEGF9 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Western Blot

1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and human brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MEGF9

MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta ‑chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31‑514) plus a 68 aa C‑terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200), followed by five EGF‑like domains (aa 204‑451). MEGF9 may run at approximately 160 kDa in SDS‑PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36‑514, human MEGF9 shares 76% aa sequence identity with mouse MEGF9.

Long Name

Multiple EGF-like-domains 9

Alternate Names

EGFL5

Entrez Gene IDs

1955 (Human); 230316 (Mouse); 313270 (Rat)

Gene Symbol

MEGF9

UniProt

Q9H1U4 (Human)

Additional MEGF9 Products

Product Documents for Human MEGF9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MEGF9 Antibody

For research use only

Related Research Areas

Neuronal Lineage Markers

Citations for Human MEGF9 Antibody

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Protocols

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