Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all mGluR3 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human mGluR3
Asp25-Ser507
Accession # Q14832

Specificity

Detects human mGluR3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) mGluR2, rhmGluR4, rhmGluR8 is observed, and less than 1% cross-reactivity with rhmGluR1, rhmGluR5, and rhmGluR7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human mGluR3 Antibody

mGluR3 antibody in Human Brain by Immunohistochemistry (IHC-P).

mGluR3 in Human Brain.

mGluR3 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Sheep Anti-Human mGluR3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4668) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human mGluR3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate putamen) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human mGluR3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR3

Metabotropic glutamate receptor 3 (mGluR3) is a 90-100 kDa, 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. It is a presynaptic receptor expressed on both neurons and glia, whose activation reduces adenylate cyclase activity. Mature human mGluR3 is 857 amino acids in length and contains a 554 amino acid (aa) N-terminal extracellular domain (ECD) (aa 23-576). The ECD binds glutamate and forms homodimers. There is one alternative splice form that is soluble, 515 aa in length and shows a 96 aa substitution for aa 442-879. Over aa 25-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3 and 67% aa sequence identity with hGluR2.

Long Name

Metabotropic Glutamate Receptor 3

Alternate Names

GPRC1C, GRM3

Entrez Gene IDs

2913 (Human); 108069 (Mouse); 24416 (Rat)

Gene Symbol

GRM3

UniProt

Q14832

Additional mGluR3 Products

Product Documents for Human mGluR3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human mGluR3 Antibody

For research use only

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Protocols

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FAQs for Human mGluR3 Antibody

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  • Q: What was the antigen retrieval method for this product?

    A: We used basic pH antigen retrieval (citrate buffer) with primary antibody incubation overnight at 4 degrees C at 10ug/mL.

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