Human MMP-2 DuoSet ELISA

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Ancillary Products Available
Human MMP-2 ELISA Standard Curve
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Product Details
Citations (18)
Supplemental Products
Reviews (3)

Human MMP-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
0.6 - 20 ng/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MMP-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Human MMP-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-2

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 2
Entrez Gene IDs:
4313 (Human); 17390 (Mouse)
Alternate Names:
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Assay Procedure


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human MMP-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation.
    Authors: Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S
    Int J Mol Sci, 2022-08-29;23(17):.
    Species: Human
    Sample Types: Tissue Culture Supernates
  2. Mg-based materials diminish tumor spreading and cancer metastases
    Authors: P Globig, R Madurawala, R Willumeit-, F Martini, E Mazzoni, BJC Luthringer
    Bioactive materials, 2022-05-10;19(0):594-610.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Role of Betaglycan in TGF-beta Signaling and Wound Healing in Human Endometriotic Epithelial Cells and in Endometriosis
    Authors: AN Mwaura, MA Riaz, JB Maoga, E Mecha, COA Omwandho, G Scheiner-B, I Meinhold-H, L Konrad
    Biology, 2022-03-26;11(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. A microphysiological model of human trophoblast invasion during implantation
    Authors: JY Park, S Mani, G Clair, HM Olson, VL Paurus, CK Ansong, C Blundell, R Young, J Kanter, S Gordon, AY Yi, M Mainigi, DD Huh
    Nature Communications, 2022-03-15;13(1):1252.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Antibody microarray analysis of amniotic fluid proteomes in women with cervical insufficiency and short cervix, and their association with pregnancy latency length
    Authors: S Hong, KH Park, YE Lee, JE Lee, YM Kim, E Joo, I Cho
    PLoS ONE, 2022-02-07;17(2):e0263586.
    Species: Human
    Sample Types: Amniotic Fluid
  6. MMPs and TIMPs levels are correlated with anthropometric parameters, blood pressure, and endothelial function in obesity
    Authors: S Boumiza, K Chahed, Z Tabka, MP Jacob, X Norel, G Ozen
    Scientific Reports, 2021-10-08;11(1):20052.
    Species: Human
    Sample Types: Plasma
  7. The Value of Fetuin-A as a Predictor to Identify Takotsubo Patients at Risk of Cardiovascular Events
    Authors: A Topf, M Mirna, N Bacher, V Paar, C Edlinger, LJ Motloch, S Gharibeh, M Bannehr, UC Hoppe, M Lichtenaue
    Journal of cardiovascular development and disease, 2021-10-05;8(10):.
    Species: Human
    Sample Types: Serum
  8. Microfluidic Single-Cell Proteomics Assay Chip: Lung Cancer Cell Line Case Study
    Authors: Y Jung, M Son, YR Nam, J Choi, JR Heath, S Yang
    Micromachines, 2021-09-23;12(10):.
    Species: Human
    Sample Types: Cell Lysates
  9. ADAMTS-5 Decreases in Coronary Arteries and Plasma from Patients with Coronary Artery Disease
    Authors: Z Wang, D Ye, J Ye, M Wang, J Liu, H Jiang, Y Xu, J Zhang, J Chen, J Wan
    Dis. Markers, 2019-12-16;2019(0):6129748.
    Species: Human
    Sample Types: Plasma
  10. Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants
    Authors: YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park
    Invest. Ophthalmol. Vis. Sci., 2019-09-03;60(12):3813-3820.
    Species: Human
    Sample Types: Plasma
  11. Glycosaminoglycans influence enzyme activity of MMP2 and MMP2/TIMP3 complex formation - Insights at cellular and molecular level
    Authors: G Ruiz-Gómez, S Vogel, S Möller, MT Pisabarro, U Hempel
    Sci Rep, 2019-03-20;9(1):4905.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing
    Authors: U Hopfner, MM Aitzetmuel, P Ne beta bach, MS Hu, HG Machens, ZN Maan, D Duscher
    Stem Cells Int, 2018-12-19;2018(0):1353085.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Novel oncogenic and chemoresistance-inducing functions of resistin in ovarian cancer cells require miRNAs-mediated induction of epithelial-to-mesenchymal transition
    Authors: L Qiu, GF Zhang, L Yu, HY Wang, XJ Jia, TJ Wang
    Sci Rep, 2018-08-21;8(1):12522.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes
    Authors: TT Vuong, SB Rønning, TAE Ahmed, K Brathagen, V Høst, MT Hincke, HP Suso, ME Pedersen
    PLoS ONE, 2018-08-06;13(8):e0201975.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. SH003 reverses drug resistance by blocking signal transducer and activator of transcription 3 (STAT3) signaling in breast cancer cells
    Authors: HS Seo, JM Ku, HJ Lee, JK Woo, C Cheon, M Kim, BH Jang, YC Shin, SG Ko
    Biosci. Rep., 2017-11-15;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Periodontitis and type 2 diabetes among women with previous gestational diabetes: epidemiological and immunological aspects in a follow-up of three years
    Authors: RP Esteves Li, LO Cota, TA Silva, SC Cortelli, JR Cortelli, FO Costa
    J Appl Oral Sci, 2017-03-01;25(2):130-139.
    Species: Human
    Sample Types: Saliva
  17. TLR ligands stimulation protects MSC from NK killing.
    Authors: Giuliani M, Bennaceur-Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, Durrbach A, Lataillade J
    Stem Cells, 2014-01-01;32(1):290-300.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Stromal fibroblasts in the microenvironment of gastric carcinomas promote tumor metastasis via upregulating TAGLN expression.
    Authors: Yu B, Chen X, Li J, Qu Y, Su L, Peng Y, Huang J, Yan J, Yu Y, Gu Q, Zhu Z, Liu B
    BMC Cell Biol, 2013-03-19;14(0):17.
    Species: Human
    Sample Types: Cell Culture Supernates


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Reviews for Human MMP-2 DuoSet ELISA

Average Rating: 4.7 (Based on 3 Reviews)

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Human MMP-2 DuoSet ELISA
By Anonymous on 03/16/2023
Sample Tested: EDTA Plasma,Urine

Tested urine at 2-fold and 10-fold. All urine samples were below the standard curve range at both dilutions. Tested plasma at 30-fold and 60-fold. All plasma samples were in range at both dilutions.

Human MMP-2 DuoSet ELISA
By Anonymous on 06/02/2020
Sample Tested: EDTA Plasma

We also ran human EDTA-plasma at a 1:30 dilution. High precision and with a low intra-assay coefficient of variation (2.0%)

Human MMP-2 DuoSet ELISA
By Jonatan Dereke on 07/02/2019
Sample Tested: EDTA Plasma

We ran human EDTA-plasma at a 1:30 dilution for this analysis with high precision