Aminopeptidase PILS/ARTS1 Antibody (6H9)

R&D Systems | Catalog # MAB2334

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6H9
View all Aminopeptidase PILS/ARTS1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 9-derived recombinant human ARTS1
Accession # Q9NZ08

Specificity

Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse Aminopeptidase PILS/ARTS1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Aminopeptidase PILS/ARTS1 Antibody (6H9)

Detection of Human Aminopeptidase PILS/ARTS1 antibody by Western Blot.

Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Aminopeptidase PILS/ARTS1 antibody by Simple WesternTM.

Detection of Human Aminopeptidase PILS/ARTS1 by Simple WesternTM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 114 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Aminopeptidase PILS/ARTS1 Antibody (6H9)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line

Western Blot

2 µg/mL
Sample: A431 human epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB2334 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aminopeptidase PILS/ARTS1

Aminopeptidase PILS/ARTS1 is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum). ARTS1 is widely expressed and releases an N-terminal amino acid (Leu). The amino acid sequence of mouse ARTS1 is 94%, 84%, 80 and 70% identical to that of rat, human, canine, and chicken.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Alternate Names

A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP

Entrez Gene IDs

51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)

Gene Symbol

ERAP1

UniProt

Q9NZ08

Additional Aminopeptidase PILS/ARTS1 Products

Product Documents for Aminopeptidase PILS/ARTS1 Antibody (6H9)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Aminopeptidase PILS/ARTS1 Antibody (6H9)

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Aminopeptidase PILS/ARTS1 Antibody (6H9)

Customer Reviews for Aminopeptidase PILS/ARTS1 Antibody (6H9) (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22918227
    Species: Human
    Verified Customer | Posted 02/19/2015

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