Human/Mouse Aminopeptidase PILS/ARTS1 Antibody Summary
Accession # Q9NZ08
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Aminopeptidase PILS/ARTS1 by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 114 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase PILS/ARTS1
Aminopeptidase PILS/ARTS1 is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum). ARTS1 is widely expressed and releases an N-terminal amino acid (Leu). The amino acid sequence of mouse ARTS1 is 94%, 84%, 80 and 70% identical to that of rat, human, canine, and chicken.
Citations for Human/Mouse Aminopeptidase PILS/ARTS1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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ER-aminopeptidase 1 determines the processing and presentation of an immunotherapy-relevant melanoma epitope
Authors: K Textoris-T, C Cammann, P Henklein, E Topfstedt, F Ebstein, S Henze, J Liepe, F Zhao, D Schadendor, B Dahlmann, W Uckert, A Paschen, M Mishto, U Seifert
Eur. J. Immunol., 2019;0(0):.
Sample Types: Cell Lysa
Applications: Western Blot
The proteasome immunosubunits, PA28 and ER-aminopeptidase 1 protect melanoma cells from efficient MART-126-35 -specific T-cell recognition.
Authors: Keller M, Ebstein F, Burger E, Textoris-Taube K, Gorny X, Urban S, Zhao F, Dannenberg T, Sucker A, Keller C, Saveanu L, Kruger E, Rothkotter H, Dahlmann B, Henklein P, Voigt A, Kuckelkorn U, Paschen A, Kloetzel P, Seifert U
Eur J Immunol, 2015;45(12):3257-68.
Sample Types: Cell Lysates
Applications: Western Blot
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