Detection of Human/Mouse BCAP by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, Daudi human Burkitt's lymphoma cell line, and L1.2 mouse pro‑B cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse BCAP Monoclonal Antibody (Catalog # MAB4857) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BCAP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BCAP (B cell adaptor for phosphoinositide 3-kinase (PI3K)), also known as PI3KAP1, participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K. BCAP null mice have deficient immune function, with B cells that exhibit enhanced apoptosis.
B Cell Adaptor Protein
Entrez Gene IDs:
118788 (Human); 83490 (Mouse); 294048 (Rat)
1810044J04Rik; B cell adaptor protein; BCAPB-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; FLJ35564; phosphoinositide 3-kinase adapter protein 1; phosphoinositide-3-kinase adaptor protein 1; Pik3ap1; RP11-34E5.3