Human/Mouse BCAP Antibody

Detection of Human BCAP by Western Blot.

Western Blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human/Mouse BCAP Monoclonal Antibody (Catalog # MAB4857) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for BCAP at approximately 80 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse BCAP/PIK3AP1 by Western Blot

miR-486 directly regulate post-transcriptional expression of BCAP. (A,B) A549 and LLC cells were treated as described in Fig. 2C. Expression levels of BCAP were detected by qRT-PCR. (C) Luciferase reporters containing wild-type (WT) or mutant (MUT) miR-486 binding sites in the BCAP 3′-UTR were co-transfected into 293 T cells along with control luciferase reporter plasmid, miR-NC, miR-486 mimic for 48 hours. (D–E) qRT-PCR analysis of BCAP mRNA levels and Western blot analysis of BCAP protein levels in A549 cell treated with miR-486 mimic. (F) A549 and LLC cells were co-transfected with GFP-LC3 plasmid, siNC or siBCAP for 48 h. Number of autophagosomes was quantified. (G) A549 and LLC cells were transfected with GFP-LC3 plasmid, miR-NC, miR-486 mimic or co-transfected with miR-486 mimic and BCAP plasmid. Number of autophagosomes was quantified. (H) A549 and LLC cells transfected with siNC or siBCAP for 48 h. Western blot analysis of phospho-AKT, AKT, phospho-mTOR, mTOR and GAPDH expression. (I) A549 or LLC cells transfected with miR-NC, miR-486 mimic or co-transfected with miR-486 and BCAP plasmid. Western blot analysis of phospho-AKT, AKT, phospho-mTOR, mTOR and GAPDH expression. All error bars indicate mean ± SEM. Experiments were repeated three times independently. **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28924214), licensed under a CC-BY license. Not internally tested by R&D Systems.