Human/Mouse Bcl-x Antibody

(6 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Bcl‑x in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    KLH-coupled Bcl-x synthetic peptide
    CSAINGNPSWHLADSPAVNGA
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.3 µg/mL
    See below
  • Simple Western
    3 µg/mL
    See below
  • Immunoprecipitation
    5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Bcl‑x by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104 cells, lane 1 and 2.5 x 104 cells, lane 2) and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl‑x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunoprecipitation
Immunoprecipitation of Human Bcl-x.

Bcl-x was immunoprecipitated from lysates (7 x 105 cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno­precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.

Detection of Human and Mouse Bcl‑x by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-x
The peptide corresponds to amino acids 49-68 of human and mouse, long and short forms of Bcl-x. The sequence used for immunization differs significantly from the sequences of the corresponding region of other members of the Bcl-2 family. Cysteine was added to the amino-terminal for coupling to KLH and for coupling to an affinity matrix.
  • Long Name:
    B Cell Lymphoma/Leukemia x
  • Entrez Gene IDs:
    598 (Human); 12048 (Mouse); 24888 (Rat)
  • Alternate Names:
    BCL2L1; Bclx; Bcl-x
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Species
Applications
Sample Type
  1. Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint.
    Authors: Schmitt E, Beauchemin M, Bertrand R
    Oncogene, 2007;26(40):5851-65.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  2. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Authors: Sade H, Krishna S, Sarin A
    J. Biol. Chem., 2004;279(4):2937-44.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.
    Authors: Armstrong T, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP
    Clin. Cancer Res., 2004;10(21):7427-37.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  4. Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.
    Authors: Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
    Clin. Cancer Res., 2004;10(6):2100-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.
    Authors: Raman VS, Akondy RS, Rath S, Bal V, George A
    J. Immunol., 2003;171(11):5876-81.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.
    Authors: Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
    J. Exp. Med., 2002;196(1):87-95.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Antibody

WB AF008 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse Bcl-x Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information