Human/Mouse Bcl-x Antibody

Catalog # Availability Size / Price Qty
AF800
AF800-SP
Detection of Human and Mouse Bcl‑x by Western Blot.
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Product Details
Citations (6)
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Human/Mouse Bcl-x Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Bcl‑x in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled Bcl-x synthetic peptide
CSAINGNPSWHLADSPAVNGA
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.3 µg/mL
See below
Simple Western
3 µg/mL
See below
Immunoprecipitation
5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse Bcl‑x by Western Blot. View Larger

Detection of Human and Mouse Bcl‑x by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104 cells, lane 1 and 2.5 x 104 cells, lane 2) and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl‑x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunoprecipitation Immunoprecipitation of Human Bcl-x. View Larger

Immunoprecipitation of Human Bcl-x.

Bcl-x was immunoprecipitated from lysates (7 x 105 cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno­precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.

Simple Western Detection of Human and Mouse Bcl‑x by Simple WesternTM. View Larger

Detection of Human and Mouse Bcl‑x by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-x

The peptide corresponds to amino acids 49-68 of human and mouse, long and short forms of Bcl-x. The sequence used for immunization differs significantly from the sequences of the corresponding region of other members of the Bcl-2 family. Cysteine was added to the amino-terminal for coupling to KLH and for coupling to an affinity matrix.

Long Name
B Cell Lymphoma/Leukemia x
Entrez Gene IDs
598 (Human); 12048 (Mouse); 24888 (Rat)
Alternate Names
BCL2L1; Bclx; Bcl-x

Product Datasheets

Citations for Human/Mouse Bcl-x Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint.
    Authors: Schmitt E, Beauchemin M, Bertrand R
    Oncogene, 2007;26(40):5851-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.
    Authors: Armstrong T, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP
    Clin. Cancer Res., 2004;10(21):7427-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.
    Authors: Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
    Clin. Cancer Res., 2004;10(6):2100-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Authors: Sade H, Krishna S, Sarin A
    J. Biol. Chem., 2004;279(4):2937-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.
    Authors: Raman VS, Akondy RS, Rath S, Bal V, George A
    J. Immunol., 2003;171(11):5876-81.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.
    Authors: Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
    J. Exp. Med., 2002;196(1):87-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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