Human/Mouse Bcl‑x Antibody

R&D Systems | Catalog # AF800

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Bcl-x Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled Bcl-x synthetic peptide
CSAINGNPSWHLADSPAVNGA

Specificity

Detects human and mouse Bcl‑x in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse Bcl‑x Antibody

Detection of Human and Mouse Bcl-x antibody by Western Blot.

Detection of Human and Mouse Bcl‑x by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104cells, lane 1 and 2.5 x 104cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunoprecipitation of Human Bcl-x.

Immunoprecipitation of Human Bcl-x.

Bcl-x was immunoprecipitated from lysates (7 x 105cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.
Detection of Human and Mouse Bcl‑x antibody by Simple WesternTM.

Detection of Human and Mouse Bcl‑x by Simple WesternTM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Bcl-x by Western Blot

Detection of Bcl-x by Western Blot

Necroptosis induction in parental CNE-2 and photon-resistant CNE-2-RR cells following X-rays or carbon-ion irradiation. (A) Endogenous changes of CNE-2-RR in the levels of caspase-8, PARP and other regulators of necroptosis compared with CNE-2 cells. Protein levels (mean±SD) in CNE-2-RR relative to the CNE-2 cells are presented. **P≤0.01. (B) Dose dependent phosphorylation of MLKL in X-ray irradiated cells at 48 hours. (C) Dose dependent phosphorylation of MLKL, PARP and cleaved-PARP in carbon ion irradiated cells at 48 hours. (D) Levels of phosphorylated forms MLKL and Bcl-x at 48 hours after X-ray (12 Gy) and carbon-ion (4 Gy) irradiation in HepG2 and Hep2 cells. (E) Levels of phosphorylated forms MLKL and Bcl-x at 48 hours after X-ray (4 Gy and 10 Gy) and carbon-ion (2 Gy and 4 Gy) irradiation in CNE-2 and CNE-2-RR cells. (F) Fold change in p-MLKL levels (mean ± SEM) in irradiated cells (Carbon ion: 2Gy and 4 Gy; X-ray: 4Gy and 10Gy) relative to the un-irradiated cells at 48 h. **P≤0.01, ****P≤0.0001. ns, no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33531997), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Bcl‑x Antibody

Application
Recommended Usage

Immunoprecipitation

5 µg/106 cells
Sample: A431 human epithelial carcinoma cell line, see our available Western blot detection antibodies

Simple Western

3 µg/mL
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

0.3 µg/mL
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using AF800 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bcl-x

The peptide corresponds to amino acids 49-68 of human and mouse, long and short forms of Bcl-x. The sequence used for immunization differs significantly from the sequences of the corresponding region of other members of the Bcl-2 family. Cysteine was added to the amino-terminal for coupling to KLH and for coupling to an affinity matrix.

Long Name

B Cell Lymphoma/Leukemia x

Alternate Names

BCL2L1, Bclx

Entrez Gene IDs

598 (Human); 12048 (Mouse); 24888 (Rat)

Gene Symbol

BCL2L1

Additional Bcl-x Products

Product Documents for Human/Mouse Bcl‑x Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Bcl‑x Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human/Mouse Bcl‑x Antibody

Customer Reviews for Human/Mouse Bcl‑x Antibody (1)

5 out of 5
1 Customer Rating
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4 Stars
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1 Stars
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22582944
    Species: Human
    Verified Customer | Posted 01/09/2015

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