Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
CSAINGNPSWHLADSPAVNGA
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse Bcl‑x Antibody
Detection of Human and Mouse Bcl‑x by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104cells, lane 1 and 2.5 x 104cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunoprecipitation of Human Bcl-x.
Bcl-x was immunoprecipitated from lysates (7 x 105cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.
Detection of Human and Mouse Bcl‑x by Simple WesternTM.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Bcl-x by Western Blot
Necroptosis induction in parental CNE-2 and photon-resistant CNE-2-RR cells following X-rays or carbon-ion irradiation. (A) Endogenous changes of CNE-2-RR in the levels of caspase-8, PARP and other regulators of necroptosis compared with CNE-2 cells. Protein levels (mean±SD) in CNE-2-RR relative to the CNE-2 cells are presented. **P≤0.01. (B) Dose dependent phosphorylation of MLKL in X-ray irradiated cells at 48 hours. (C) Dose dependent phosphorylation of MLKL, PARP and cleaved-PARP in carbon ion irradiated cells at 48 hours. (D) Levels of phosphorylated forms MLKL and Bcl-x at 48 hours after X-ray (12 Gy) and carbon-ion (4 Gy) irradiation in HepG2 and Hep2 cells. (E) Levels of phosphorylated forms MLKL and Bcl-x at 48 hours after X-ray (4 Gy and 10 Gy) and carbon-ion (2 Gy and 4 Gy) irradiation in CNE-2 and CNE-2-RR cells. (F) Fold change in p-MLKL levels (mean ± SEM) in irradiated cells (Carbon ion: 2Gy and 4 Gy; X-ray: 4Gy and 10Gy) relative to the un-irradiated cells at 48 h. **P≤0.01, ****P≤0.0001. ns, no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33531997), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse Bcl‑x Antibody
Immunoprecipitation
Sample: A431 human epithelial carcinoma cell line, see our available Western blot detection antibodies
Simple Western
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using AF800 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Bcl-x
Long Name
Alternate Names
Gene Symbol
Additional Bcl-x Products
Product Documents for Human/Mouse Bcl‑x Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse Bcl‑x Antibody
For research use only
Related Research Areas
Citations for Human/Mouse Bcl‑x Antibody
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Customer Reviews for Human/Mouse Bcl‑x Antibody (1)
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Application: Western BlotSample Tested: See PMID 22582944Species: HumanVerified Customer | Posted 01/09/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Associated Pathways
MAPK Signaling: Oxidative Stress Pathway
