|Detection of Human CHD1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse CHD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6195) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD1 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
CHD1 (Chromohelicase/ATPase DNA‑binding protein 1) is a 200‑220 kDa member of the SNF2/RAD54 helicase family of proteins. It is an ATP‑dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state. In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency. Human CHD1 is 1710 amino acids (aa) in length. It contains two Ser‑rich regions (aa 1‑70 and 117‑137), two Chromo (chromatin‑organizer‑modifer) domains (aa 272‑364 and 389‑452), a SNF2 family N‑terminal domain (aa 484‑763) and a C‑terminal helicase domain (aa 792‑943). There are at least 30 Ser/Thr phosphorylation sites. Over aa 1531‑1710, human CHD1 shares 92% aa identity with mouse CHD1.
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