Human/Mouse Cited-2 Antibody

(3 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse Cited-2 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Cited-2
    Met1-Cys269
    Accession # O35740
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    3-15 µg/mL
    See below
  • Immunocytochemistry
    1-10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse Cited‑2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Cited‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cited‑2 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Cited‑2 in HeLa Human Cell Line. Cited‑2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse Cited‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Cited‑2 in C2C12 Mouse Cell Line. Cited‑2 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse Cited‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Cited‑2 in NIH‑3T3 Mouse Cell Line. Cited‑2 was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Sheep Anti-Human/Mouse Cited‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Cited‑2 in Human Breast Cancer Tissue. Cited‑2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse Cited‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cited-2

Cited-2 is a 270 amino acid protein that functions by binding to the CH1 region of CREB binding protein (CBP)/p300. This binding interferes with certain transcription factors like HIF1 alpha and STAT2 blocking their ability to bind and interact with CBP/p300. Cited-2 knockout mice die due to defects in heart and neural tube development. Mutations in Cited-2 have been reported in patients with congenital heart defects. It appears Cited-2 modulates apoptosis by a p53 independent pathway whereby the details still need to be elucidated.

  • Long Name:
    Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
  • Entrez Gene IDs:
    10370 (Human); 17684 (Mouse); 114490 (Rat)
  • Alternate Names:
    Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain2,cbp/p300-interacting transactivator 2; Cited2; Cited-2; MRG-1; MRG1melanocyte-specific gene 1-related gene 1; MSG1-related gene 1; MSG-related protein 1; P35srj
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Cited2, a transcriptional modulator protein, regulates metabolism in murine embryonic stem cells.
    Authors: Li Q, Hakimi P, Liu X, Yu W, Ye F, Fujioka H, Raza S, Shankar E, Tang F, Dunwoodie S, Danielpour D, Hoppel C, Ramirez-Bergeron D, Qu C, Hanson R, Yang Y
    J Biol Chem, 2014;289(1):251-63.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ChIP
  2. Cited2 gene controls pluripotency and cardiomyocyte differentiation of murine embryonic stem cells through Oct4 gene.
    Authors: Li Q, Ramirez-Bergeron D, Dunwoodie S, Yang Y
    J Biol Chem, 2012;287(34):29088-100.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  3. HIF-1 alpha deletion partially rescues defects of hematopoietic stem cell quiescence caused by Cited2 deficiency.
    Authors: Du J, Chen Y, Li Q, Han X, Cheng C, Wang Z, Danielpour D, Dunwoodie SL, Bunting KD, Yang YC
    Blood, 2012;119(12):2789-98.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ChIP
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Antigen Retrieval Reagent-Basic

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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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