Cited-2 Antibody

R&D Systems | Catalog # AF5005

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Cited-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Cited-2
Met1-Cys269
Accession # O35740

Specificity

Detects mouse Cited-2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Cited-2 Antibody

Detection of Human and Mouse Cited-2 antibody by Western Blot.

Detection of Human and Mouse Cited‑2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cited-2 at approximately 30-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cited-2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Cited‑2 in HeLa Human Cell Line.

Cited-2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cited-2 antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

Cited‑2 in C2C12 Mouse Cell Line.

Cited-2 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cited-2 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC).

Cited‑2 in NIH‑3T3 Mouse Cell Line.

Cited-2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cited-2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Cited‑2 in Human Breast Cancer Tissue.

Cited-2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Cited-2 Antibody

Application
Recommended Usage

Immunocytochemistry

1-10 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line

Reviewed Applications

Read 1 review rated 4 using AF5005 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cited-2

Cited-2 is a 270 amino acid protein that functions by binding to the CH1 region of CREB binding protein (CBP)/p300. This binding interferes with certain transcription factors like HIF1 alpha and STAT2 blocking their ability to bind and interact with CBP/p300. Cited-2 knockout mice die due to defects in heart and neural tube development. Mutations in Cited-2 have been reported in patients with congenital heart defects. It appears Cited-2 modulates apoptosis by a p53 independent pathway whereby the details still need to be elucidated.

Long Name

Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2

Alternate Names

Cited2, MRG-1, MSG-related protein 1, P35srj

Entrez Gene IDs

10370 (Human); 17684 (Mouse); 114490 (Rat)

Gene Symbol

CITED2

UniProt

O35740

Additional Cited-2 Products

Product Documents for Cited-2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Cited-2 Antibody

For research use only

Citations for Cited-2 Antibody

Customer Reviews for Cited-2 Antibody (1)

4 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Chromatin Immunoprecipitation
    Sample Tested: See PMID 22308296
    Species: Mouse
    Verified Customer | Posted 01/08/2015

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Protocols

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FAQs

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Associated Pathways

HIF Repressor Pathways
HIF Repressor Pathway Thumbnail