Human/Mouse Desmocollin-1 Antibody

Catalog # Availability Size / Price Qty
MAB7367
MAB7367-SP
Detection of Human and Mouse Desmocollin‑1 by Western Blot.
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Citations (2)
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Human/Mouse Desmocollin-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Desmocollin-1 in ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human Desmocollin-1 is observed, and no cross-reactivity with recombinant mouse Desmocollin-2 or -3 is observed.
Source
Monoclonal Rat IgG2A Clone # 772906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-1
Arg135-Lys691
Accession # P55849
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
1-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse Desmocollin‑1 by Western Blot. View Larger

Detection of Human and Mouse Desmocollin‑1 by Western Blot. Western blot shows lysates of SK‑Mel‑28 human malignant melanoma cell line and B16‑F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Desmocollin‑1 at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Desmocollin-1 in B16‑F1 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Desmocollin-1 in B16‑F1 Mouse Cell Line by Flow Cytometry.
B16‑F1 mouse melanoma cell line was stained with Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Flow Cytometry Detection of Desmocollin‑1 in A549 Human Cell Line by Flow Cytometry. View Larger

Detection of Desmocollin‑1 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Immunocytochemistry Desmocollin‑1  in A549 Human Cell Line. View Larger

Desmocollin‑1 in A549 Human Cell Line. Desmocollin‑1 was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/Mouse
Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Desmocollin‑1  in Mouse Skin. View Larger

Desmocollin‑1 in Mouse Skin. Desmocollin‑1 was detected in perfusion fixed frozen sections of mouse skin using Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to hair root sheath. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmocollin-1

DSC-1 (Desmocollin [Greek for "glue-that-binds"]-1) is an approximately 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including the spinous and granular layers of keratinized and nonkeratinized epithelia of the oral cavity and skin.
DSC‑1 expression is induced by DSG-1. It serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, homodimeric DSC-1 may form heterotypic interactions with DSG-1 in-trans, and bind to the cytoskeleton intracellularly via plakophilin-1. Mature mouse DSC-1 is a 760 amino acid (aa) type I transmembrane glycoprotein (aa 135-691). The mature molecule contains a 557 aa extracellular region with five cadherin domains (aa 135-682), and a 172 aa cytoplasmic domain. There is one splice variant that shows an 11 aa substitution for aa 822-886. Over aa 135-691, mouse DSC‑1 shares 82% aa sequence identity with human DSC-1.

Entrez Gene IDs
1823 (Human); 13505 (Mouse); 291759 (Rat)
Alternate Names
cadherin family member 1; CDHF1; desmocollin 1; Desmocollin1; Desmocollin-1; desmosomal glycoprotein 2/3; DG2/DG3 ; DG2/DG3; DSC1

Product Datasheets

Citations for Human/Mouse Desmocollin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. BubR1 Insufficiency Impairs Liver Regeneration in Aged Mice after Hepatectomy through Intercalated Disc Abnormality
    Sci Rep, 2016;6(0):32399.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Fresh
  2. A spontaneous deletion within the desmoglein 3 extracellular domain of mice results in hypomorphic protein expression, immunodeficiency, and a wasting disease phenotype.
    Authors: Kountikov E, Poe J, Maclver N, Rathmell J, Tedder T
    Am J Pathol, 2015;185(3):617-30.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB

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