Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Draxin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Draxin
aa 26-349
Accession # Q8NBI3

Specificity

Detects human Draxin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Draxin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse Draxin Antibody

Detection of Human Draxin antibody by Western Blot.

Detection of Human Draxin by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Draxin antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC).

Draxin in SH‑SY5Y Human Cell Line.

Draxin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Draxin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Draxin in Mouse Embryo.

Draxin was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to diencephalon. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse Draxin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line

Reviewed Applications

Read 1 review rated 4 using AF6148 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Draxin

Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58 kDa, presumable glycoprotein member of the draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature human Draxin is 324 amino acids (aa) in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa 274-333). Mature human Draxin (aa 26-349) shares 79% aa identity with mature mouse Draxin.

Long Name

Dorsal Repulsive Axon Guidance Protein

Alternate Names

C1orf187, Neucrin

Entrez Gene IDs

374946 (Human); 70433 (Mouse); 691317 (Rat)

Gene Symbol

DRAXIN

UniProt

Q8NBI3 (Human)

Additional Draxin Products

Product Documents for Human/Mouse Draxin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Draxin Antibody

For research use only

Related Research Areas

Wnt Inhibitors

Citations for Human/Mouse Draxin Antibody

Customer Reviews for Human/Mouse Draxin Antibody (1)

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23038776
    Species: Other
    Verified Customer | Posted 01/08/2015

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Protocols

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