Gastrokine 1 Antibody

Detection of Gastrokine 1 by Western Blot

Gastrokine expression during pancreatic inflammation, in vitro in acinar cells and its detection in pancreatic juice.A Infiltrating inflammatory cells (*) around the PanIN lesions in KC mice. Immunohistochemical GKN1 and GKN2 positivity, while the inflammatory cells are negative (scale bars 50 µm). B H&E, GKN1, and GKN2 staining in C57BL/6 mice (scale bar 100 µm) and in different models of pancreatic inflammation (scale bars 50 µm). The histological images of cerulein treatment represents the chronic treatment regimen. C Light microscopy photos of isolated acinar cells embedded in collagen matrix from wt and KC mice at day 0 and day 4. Gkn1 and Gkn2 gene expression of KC acinar cells compared to untreated wild type acinar cells. D Gkn1 and Gkn2 protein expression on western blot in serum of 9 months old wild type and KC mice. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35082384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Gastrokine 1 by Immunohistochemistry

Gastrokine expression during pancreatic inflammation, in vitro in acinar cells and its detection in pancreatic juice.A Infiltrating inflammatory cells (*) around the PanIN lesions in KC mice. Immunohistochemical GKN1 and GKN2 positivity, while the inflammatory cells are negative (scale bars 50 µm). B H&E, GKN1, and GKN2 staining in C57BL/6 mice (scale bar 100 µm) and in different models of pancreatic inflammation (scale bars 50 µm). The histological images of cerulein treatment represents the chronic treatment regimen. C Light microscopy photos of isolated acinar cells embedded in collagen matrix from wt and KC mice at day 0 and day 4. Gkn1 and Gkn2 gene expression of KC acinar cells compared to untreated wild type acinar cells. D Gkn1 and Gkn2 protein expression on western blot in serum of 9 months old wild type and KC mice. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35082384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Gastrokine 1 by Immunohistochemistry

Gastrokine expression in KPC mice.AGkn1 and Gkn2 transcripts in 4 months old KPC mice compared to age matched wild-type mice. B Visualization of tumor and peri-tumoral tissue in 4 months old KPC mice. H&E, GKN1, and GKN2 immunohistochemistry in consecutive cuts (scale bars 100 µm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35082384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Gastrokine 1 by Immunohistochemistry

Gastrokine expression in pre-malignant lesions of KC mice and in human pancreas samples.A Selected genes from the microarray analysis of KC mice (at 4, 10, and 17 weeks of age). B Temporal analysis of Gkn1 and Gkn2 transcripts in KC mice by qRT-PCR (one-way ANOVA). C H&E, GKN1, and GKN2 staining in KC mice (scale bars 50 µm). Acini and ADM visualized in 10 weeks old; low- and high-grade PanINs in 9 months old and PDAC in 12 months old KC mice. DGKN1 and GKN2 mRNA transcripts from patients undergoing pancreatic surgery due to lesions in the pancreas compared to healthy control pancreas. Expression levels are reported as dCT (40-Ct). (χ²-test malignancy: GKN1 12.53 > 3.84, GKN2 29.77 > 3.84; adenoma: GKN1 7.79 > 3.84, GKN2 10.92 > 3.85) E H&E, GKN1, and GKN2 immunohistochemistry in human low-grade PanINs and PDAC (scale bar 100 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35082384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Gastrokine 1 by Immunohistochemistry

In vitro and in vivo effects of GKN1 protein.A Viability of Panc-02 cells was assessed by MTT colorimetric assay after 24 h of incubation with conditioned media containing GKN1 protein. B beta -Galactosidase staining on Panc-02 cells after incubation with GKN1 or control conditioned medium. Representative pictures of compared groups are shown for the 72 h time point. Expression profile of senescence genes such as p16 and p21 was performed via qPCR analysis on Panc-02 cells treated with conditioned media with or without GKN1. Gene expression was analyzed over 24, 48, and 72 h. C Migration assay of Panc-02 cells after 24 h of incubation with GKN1 or control conditioned medium. Representative pictures at 5x magnification show migration over 1, 10, and 24 h time period (scale bars 200 μm). D In vitro transdifferentiation of isolated acinar cells from KC mice, treated with conditioned media containing GKN1. E Subcutaneous tumor development over 19 days after injection of either Panc-02-empty vector or Gastrokine1+ Panc-02 cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35082384), licensed under a CC-BY license. Not internally tested by R&D Systems.