Human/Mouse Gastrokine 1 Antibody

Catalog # Availability Size / Price Qty
AF7287
AF7287-SP
Detection of Mouse and Rat Gastrokine 1 by Western Blot.
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Human/Mouse Gastrokine 1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Gastrokine 1 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Gastrokine 1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Gastrokine 1
Tyr38-Tyr201
Accession # Q9CR36
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat Gastrokine 1 antibody by Western Blot. View Larger

Detection of Mouse and Rat Gastrokine 1 by Western Blot. Western blot shows lysates of mouse stomach tissue and rat stomach tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7287) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Gastrokine 1 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Gastrokine 1 antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

Gastrokine 1 in Human Stomach. Gastrokine 1 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Mouse Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7287) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Gastrokine 1 antibody in Mouse Stomach by Immunohistochemistry (IHC-Fr). View Larger

Gastrokine 1 in Mouse Stomach. Gastrokine 1 was detected in perfusion fixed frozen sections of mouse stomach using Sheep Anti-Mouse Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7287) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to islets. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gastrokine 1

Gastrokine-1 (GKN1; also CA11 and AMP-18) is an 18-20 kDa member of the Gastrokine protein family. It has limited expression, being restricted to mucous secreting pyloric antrum epithelial cells. Gastrokine-1 appears to promote epithelial cell proliferation and migration, and induce the formation of tight junctions between epithelial cells. By contrast, gastrokine-1 induces Fas expression in tumor cells, resulting in apoptosis. Mature mouse gastrokine-1 is 166 amino acids (aa) in length (aa 36-201). Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 71-165) that contains a mitogenic sequence (aa 113-131). There is one potential alternative start site at Met18. Over aa 38-201, mouse gastrokine-1 shares 65% and 92% aa sequence identity with human and rat gastrokine-1, respectively.

Long Name
Take out Column D name
Entrez Gene IDs
56287 (Human); 66283 (Mouse); 297418 (Rat)
Alternate Names
18 kDa antrum mucosa protein; AMP18; AMP-18; AMP18BRICHOS domain containing 1; BRICD1; CA11; CA11MGC70354; FOV; Foveolin; Gastrokine 1; gastrokine-1; GKN1; Protein CA11

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