hh Human/Mouse IGF-I R Antibody AF-305-NA: R&D Systems

Human/Mouse IGF-I R Antibody

(8 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse IGF-I R in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant mouse IGF-I R is observed. In direct ELISAs, less than 1% cross-reactivity with recombinant human IGF‑II R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    recombinant human IGF-I R extracellular domain
    Accession # P08069
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IGF-I R (Catalog # 391-GR) and recombinant Mouse IGF-I R.
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    1-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 6 ng/mL Recombinant Human IGF‑I.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IGF‑I R in MCF‑7 and HDLM-2 Human Cell Lines. IGF‑I R was detected in immersion fixed MCF‑7 human breast cancer cell line (left panel) and HDLM‑2 human Hodgkin's lymphoma cell line (right panel) using Goat Anti-Human/Mouse IGF‑I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocols for Fluorescent ICC Staining of Cells on Coverslips and Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
IGF-I R in Mouse Embryo. IGF-I R was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 10 µg/mL overnight at
4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
IGF‑I R in Human Placenta. IGF‑I R was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 15 µg/mL Goat Anti-Human IGF‑I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑305‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF‑I R Antibody. Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA). The ND50 is typically 0.5-1.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I R
IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.
  • References:
    1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.
  • Long Name:
    Insulin-like Growth Factor I Receptor
  • Entrez Gene IDs:
    3480 (Human)
  • Alternate Names:
    CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies
    Authors: J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo
    Cancer Res, 2017;0(0):.
    Species: Rat
    Sample Type: Whole Cells
    Application: Functional Assay
  2. Mycobacterium leprae-induced Insulin-like Growth Factor I attenuates antimicrobial mechanisms, promoting bacterial survival in macrophages
    Authors: L R Batista-Si
    Sci Rep, 2016;6(0):27632.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Luciferase Assay
  3. Transplantation of Human Neural Progenitor Cells Expressing IGF-1 Enhances Retinal Ganglion Cell Survival.
    Authors: Ma J, Guo C, Guo C, Sun Y, Liao T, Beattie U, Lopez F, Chen D, Lashkari K
    PLoS ONE, 2015;10(4):e0125695.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. LMP1 promotes expression of insulin-like growth factor 1 (IGF1) to selectively activate IGF1 receptor and drive cell proliferation.
    Authors: Tworkoski K, Raab-Traub N
    J Virol, 2015;89(5):2590-602.
    Species: Human
    Sample Type: Cell Lysates
    Application: Neut
  5. IGF-IR mediated mammary tumorigenesis is enhanced during pubertal development.
    Authors: Jones R, Watson K, Campbell C, Moorehead R
    PLoS ONE, 2014;9(9):e108781.
  6. FGFR2 is amplified in the NCI-H716 colorectal cancer cell line and is required for growth and survival.
    Authors: Mathur, Anjili, Ware, Christop, Davis, Lenora, Gazdar, Adi, Pan, Bo-Sheng, Lutterbach, Bart
    PLoS ONE, 2014;9(6):e98515.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
    Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
    Cancer Res., 2009;69(7):2996-3003.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Differential expression of receptor tyrosine kinases (RTKs) and IGF-I pathway activation in human uterine leiomyomas.
    Authors: Yu L, Saile K, Swartz CD, He H, Zheng X, Kissling GE, Di X, Lucas S, Robboy SJ, Dixon D
    Mol. Med., 2008;14(5):264-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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