Human/Mouse IL-5 Antibody

Catalog #: MAB405R
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB405R-100
MAB405R-SP
MAB405R-500
MAB405R-01M

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Cell Proliferation Induced by IL-5 and Neutralization by Human/Mouse IL-5 Antibody.

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All IL-5 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IL-5 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.

Source

Recombinant Monoclonal Rat IgG₁ Clone # TRFK5R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse T cell clone-derived partially purified recombinant mouse IL-5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human IL-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human IL‑5 Biotinylated Antibody (Catalog # BAM6051)

Standard: Recombinant Human IL-5 Protein (Catalog # 205-IL)

Mouse IL-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse IL‑5 Biotinylated Antibody (Catalog # BAM705)

Standard: Recombinant Mouse IL-5 Protein (Catalog # 405-ML)

Neutralization

Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 4-15 ng/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL-5 and Neutralization by Human/Mouse IL-5 Antibody. Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405R). The ND₅₀ is typically 4-15 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Long Name

Interleukin 5

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 100034199 (Equine); 493803 (Feline)

Alternate Names

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse IL-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1.
Authors: Kankaanranta H, Ilmarinen P, Zhang X, Adcock I, Lahti A, Barnes P, Giembycz M, Lindsay M, Moilanen E
PLoS ONE, 2014-02-28;9(2):e90298.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization