Human/Mouse IL-5 Antibody MAB405R-100: R&D Systems

Human/Mouse IL-5 Antibody

Recombinant Monoclonal Antibody.
(1 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
  • Source
    Recombinant Monoclonal Rat IgG1 Clone # TRFK5R
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Mouse T cell clone-derived partially purified recombinant mouse IL-5
  • Formulation
    Supplied as a solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Human IL-5 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Mouse IL‑5 Antibody (Catalog # MAB405R)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human IL‑5 Biotinylated Antibody (Catalog # BAM6051)
  • ELISA Standard
     
    Recombinant Human IL-5 Protein (Catalog # 205-IL)
    • Mouse IL-5 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Mouse IL‑5 Antibody (Catalog # MAB405R)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Mouse IL‑5 Biotinylated Antibody (Catalog # BAM705)
  • ELISA Standard
     
    Recombinant Mouse IL-5 Protein (Catalog # 405-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 4-15 ng/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL-5 and Neutralization by Human/Mouse IL-5 Antibody. Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405R). The ND50 is typically 4-15 ng/mL.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

  • Long Name:
    Interleukin 5
  • Entrez Gene IDs:
    3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
  • Alternate Names:
    BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1.
    Authors: Kankaanranta H, Ilmarinen P, Zhang X, Adcock I, Lahti A, Barnes P, Giembycz M, Lindsay M, Moilanen E
    PLoS ONE, 2014;9(2):e90298.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Isotype Control

Ctrl MAB005 37  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG1 Isotype Control

Ctrl MAB005R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse IL-5 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information