Mouse IL-5 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM705
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews

Mouse IL-5 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-5, recombinant mouse (rm) IL‑3, or rmGM-CSF is observed.
Source
Monoclonal Rat IgG2A Clone # TRFK4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse T cell clone-derived partially purified recombinant mouse IL-5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Mouse IL-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human/Mouse IL‑5 Antibody (Catalog # MAB405)

Capture Reagent: Human/Mouse IL‑5 Antibody (Catalog # MAB405R)

Standard: Recombinant Mouse IL-5 Protein (Catalog # 405-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentiation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5-specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

Product Datasheets

Citations for Mouse IL-5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
    Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
    J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  3. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006;168(6):1940-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  4. The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
    Authors: Nygaard UC, Ormstad H, Aase A, Lovik M
    Toxicology, 2005;206(2):181-93.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002;160(4):1353-60.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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