Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # TRFK4
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Product Specifications

Immunogen

Mouse T cell clone-derived partially purified recombinant mouse IL-5

Specificity

Detects mouse IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-5, recombinant mouse (rm) IL‑3, or rmGM-CSF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse IL‑5 Biotinylated Antibody

Mouse IL-5 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Mouse IL‑5 Antibody (Catalog # MAB405)
  • Capture Reagent: Human/Mouse IL‑5 Antibody (Catalog # MAB405R)
  • Standard: Recombinant Mouse IL-5 Protein (Catalog # 405-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentiation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5-specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 100034199 (Equine); 493803 (Feline)

Gene Symbol

IL5

Additional IL-5 Products

Product Documents for Mouse IL‑5 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL‑5 Biotinylated Antibody

For research use only

Citations for Mouse IL‑5 Biotinylated Antibody

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