ING1 Antibody (585915)

R&D Systems | Catalog # MAB5758

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 585915
View all ING1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ING1
Arg70-Ala184
Accession # Q9UK53

Specificity

Detects human and mouse ING1 in Western blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ING1 Antibody (585915)

Detection of Human and Mouse ING1 antibody by Western Blot.

Detection of Human and Mouse ING1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ING1 Monoclonal Antibody (Catalog # MAB5758) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ING1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for ING1 Antibody (585915)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and HT‑2 mouse T cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ING1

ING1 (inhibitor of growth-1) is a member of the ING family of tumor suppressive and apoptosis-promoting epigenetic regulators of chromatin structure. They interact with and alter the ratio of pro- and anti-apoptotic Bcl-2 family members. The p53 tumor suppressor and its downstream targets can potentiate ING1-induced apoptosis. Human isoforms p33ING1, p24ING1c, p33ING1b and p47ING1a vary in N-terminal sequence, resulting in 279, 210, 235 and 422 aa proteins, respectively. The sequence used as an immunogen is common to all isoforms, and shares 83% and 81% amino acid (aa) identity with mouse and rat ING1, respectively.

Long Name

Inhibitor of Growth 1

Alternate Names

p24ING1c, p33ING1b, p47ING1a

Entrez Gene IDs

3621 (Human); 26356 (Mouse); 306626 (Rat)

Gene Symbol

ING1

UniProt

Q9UK53 (Human)

Additional ING1 Products

Product Documents for ING1 Antibody (585915)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for ING1 Antibody (585915)

For research use only

Related Research Areas

Cancer Biomarkers
p53 Pathway

Citations for ING1 Antibody (585915)

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