Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 318311
View all Kirrel2/NEPH3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kirrel2
Gly21-Arg503
Accession # Q6UWL6

Specificity

Detects human and mouse Kirrel2/NEPH3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human/Mouse Kirrel2/NEPH3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Kirrel2 (Catalog # 2564-K2)
Recombinant Mouse Kirrel2 (Catalog # 2930-K2)
under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kirrel2/NEPH3

Kirrel2, also known as nephrin-like 3, nephrin-like gene 1 and filtrin, is a type I transmembrane protein belonging to the immunoglobulin superfamily. By alternative splicing, at least 5 isoforms that differ in their extracellular and/or intracellular regions have been described. Kirrel2 is expressed in the pancreas, lymph nodes, kidney glomerulus, as well as brain. The extracellular domains of human and mouse Kirrel2 share 86% amino acid sequence identity.

Long Name

Kin of Irregular Chiasm-like 2, Splice Variant A

Alternate Names

Filtrin, NEPH3, NLG1

Entrez Gene IDs

84063 (Human); 243911 (Mouse)

Gene Symbol

KIRREL2

UniProt

Q6UWL6

Additional Kirrel2/NEPH3 Products

Product Documents for Human/Mouse Kirrel2/NEPH3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Kirrel2/NEPH3 Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

Citations for Human/Mouse Kirrel2/NEPH3 Antibody

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