Detection of Human Lumican by Western Blot. Western blot shows lysates of human colon tissue and human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Lumican Monoclonal Antibody (Catalog # MAB2846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lumican is a secreted matrix protein that belongs to the small
leucine-rich proteoglycan (SLRP) class II family. Mature Lumican has a
negatively charged N-terminal domain that contains tyrosine sulfates,
followed by twelve leucine-rich repeats, which are flanked by cysteine
loops. Lumican is expressed widely in mammalian connective tissues,
while corneal Lumican exists as a keratan sulfate proteoglycan in adult
cartilage, Lumican exists primarily as a glycoprotein and lacks keratan
sulfate. The amino acid sequence of human Lumican shares 88% amino acid
sequence identity with that of mature mouse Lumican.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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