Human/Mouse Lumican Antibody

Catalog # Availability Size / Price Qty
Detection of Human Lumican by Western Blot.
1 Image
Product Details
Citations (2)
Supplemental Products

Human/Mouse Lumican Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse Lumican in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 358022
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Lumican
Accession # P51884
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Lumican antibody by Western Blot. View Larger

Detection of Human Lumican by Western Blot. Western blot shows lysates of human colon tissue and human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Lumican Monoclonal Antibody (Catalog # MAB2846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lumican

Lumican is a secreted matrix protein that belongs to the small leucine-rich proteoglycan (SLRP) class II family. Mature Lumican has a negatively charged N-terminal domain that contains tyrosine sulfates, followed by twelve leucine-rich repeats, which are flanked by cysteine loops. Lumican is expressed widely in mammalian connective tissues, while corneal Lumican exists as a keratan sulfate proteoglycan in adult cartilage, Lumican exists primarily as a glycoprotein and lacks keratan sulfate. The amino acid sequence of human Lumican shares 88% amino acid sequence identity with that of mature mouse Lumican.

Entrez Gene IDs
4060 (Human); 17022 (Mouse)
Alternate Names
KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican

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Citations for Human/Mouse Lumican Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A Proteomic Approach for Understanding the Mechanisms of Delayed Corneal Wound Healing in Diabetic Keratopathy Using Diabetic Model Rat
    Authors: T Yamamoto, H Otake, N Hiramatsu, N Yamamoto, A Taga, N Nagai
    Int J Mol Sci, 2018;19(11):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Regulation of corneal inflammation by neutrophil-dependent cleavage of keratan sulfate proteoglycans as a model for breakdown of the chemokine gradient.
    Authors: Carlson EC, Sun Y, Auletta J
    J. Leukoc. Biol., 2010;88(3):517-22.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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