Human/Mouse PARP Antibody
Human/Mouse PARP Antibody Summary
Val71-Pro329
Accession # NP_031441
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Human PARP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated with anti-Fas and 1 µM staurosporine (STS) for 0 to 4 hours (h; as indicated). PVDF membrane was probed with 0.6 µg/mL of Rat Anti-Human/Mouse PARP Monoclonal Antibody (Catalog # MAB600) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PARP at approximately 116 kDa and the 84 kDa product of PARP cleavage (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Mouse PARP by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line treated with 1 µM staurosporine (STS) for 0 to 4 hours (h; as indicated). PVDF membrane was probed with 0.6 µg/mL of Rat Anti-Human/Mouse PARP Monoclonal Antibody (Catalog # MAB600) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PARP at approximately 116 kDa and the 60-70 kDa bands generated with staurosporine treatment (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PARP
PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
Product Datasheets
Citations for Human/Mouse PARP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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The Molecular Basis of Tight Nuclear Tethering and Inactivation of cGAS
Authors: B Zhao, P Xu, CM Rowlett, T Jing, O Shinde, Y Lei, AP West, WR Liu, P Li
Nature, 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
RNA stability regulates human T cell leukemia virus type 1 gene expression in chronically-infected CD4 T cells
Authors: HC Lin, PJ Simon, RM Ysla, SL Zeichner, G Brewer, AB Rabson
Virology, 2017;508(0):7-17.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Conditional deletion of Nbs1 in murine cells reveals its role in branching repair pathways of DNA double-strand breaks.
Authors: Yang YG, Saidi A, Frappart PO, Barrucand C, Dumon-Jones V, Michelon J, Herceg Z
EMBO J., 2006;25(23):5527-38.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Distinct promoters mediate constitutive and inducible Bcl-XL expression in malignant lymphocytes.
Authors: Habens F, Lapham AS, Dallman CL, Pickering BM, Michels J, Marcusson EG, Johnson PW, Packham G
Oncogene, 2006;26(13):1910-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Transcriptional repressor activating transcription factor 3 protects human umbilical vein endothelial cells from tumor necrosis factor-alpha-induced apoptosis through down-regulation of p53 transcription.
Authors: Kawauchi J, Zhang C, Nobori K, Hashimoto Y, Adachi MT, Noda A, Sunamori M, Kitajima S
J. Biol. Chem., 2002;277(41):39025-34.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot
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Secondary Antibodies
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