Human/Mouse PARP Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse PARP in Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 53015
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse PARP
    Val71-Pro329
    Accession # NP_031441
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.6 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PARP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated with anti‑Fas and 1 µM staurosporine (STS) for 0 to 4 hours (h; as indicated). PVDF membrane was probed with 0.6 µg/mL of Rat Anti-Human/Mouse PARP Monoclonal Antibody (Catalog # MAB600) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PARP at approximately 116 kDa and the 84 kDa product of PARP cleavage (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Mouse PARP by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line treated with 1 µM staurosporine (STS) for 0 to 4 hours (h; as indicated). PVDF membrane was probed with 0.6 µg/mL of Rat Anti-Human/Mouse PARP Monoclonal Antibody (Catalog # MAB600) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PARP at approximately 116 kDa and the 60-70 kDa bands generated with staurosporine treatment (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PARP
PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
  • Long Name:
    Poly [ADP-ribose] Polymerase
  • Entrez Gene IDs:
    142 (Human); 11545 (Mouse)
  • Alternate Names:
    ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Species
Applications
Sample Type
  1. Distinct promoters mediate constitutive and inducible Bcl-XL expression in malignant lymphocytes.
    Authors: Habens F, Lapham AS, Dallman CL, Pickering BM, Michels J, Marcusson EG, Johnson PW, Packham G
    Oncogene, 2006;26(13):1910-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Conditional deletion of Nbs1 in murine cells reveals its role in branching repair pathways of DNA double-strand breaks.
    Authors: Yang YG, Saidi A, Frappart PO, Barrucand C, Dumon-Jones V, Michelon J, Herceg Z
    EMBO J., 2006;25(23):5527-38.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Transcriptional repressor activating transcription factor 3 protects human umbilical vein endothelial cells from tumor necrosis factor-alpha-induced apoptosis through down-regulation of p53 transcription.
    Authors: Kawauchi J, Zhang C, Nobori K, Hashimoto Y, Adachi MT, Noda A, Sunamori M, Kitajima S
    J. Biol. Chem., 2002;277(41):39025-34.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2A Isotype Control

Ctrl MAB006R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Human/Mouse PARP Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human/Mouse PARP Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Growing cells - tissue culture.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
Growing cells - tissue culture
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Growing cells - tissue culture Human Anonymous 05/16/2017
Show

Summary

ApplicationGrowing cells - tissue culture
Sample TestedUmbilical vein endothelial cells
SpeciesHuman

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information