Human/Mouse PARP Antibody

Catalog # Availability Size / Price Qty
MAB600
MAB600-SP
Detection of Human PARP by Western Blot.
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse PARP Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse PARP in Western blots.
Source
Monoclonal Rat IgG2A Clone # 53015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse PARP
Val71-Pro329
Accession # NP_031441
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.6 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human PARP antibody by Western Blot. View Larger

Detection of Human PARP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated with anti-Fas and 1 µM staurosporine (STS) for 0 to 4 hours (h; as indicated). PVDF membrane was probed with 0.6 µg/mL of Rat Anti-Human/Mouse PARP Monoclonal Antibody (Catalog # MAB600) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PARP at approximately 116 kDa and the 84 kDa product of PARP cleavage (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot Detection of Mouse PARP antibody by Western Blot. View Larger

Detection of Mouse PARP by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line treated with 1 µM staurosporine (STS) for 0 to 4 hours (h; as indicated). PVDF membrane was probed with 0.6 µg/mL of Rat Anti-Human/Mouse PARP Monoclonal Antibody (Catalog # MAB600) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PARP at approximately 116 kDa and the 60-70 kDa bands generated with staurosporine treatment (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PARP

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Long Name
Poly [ADP-ribose] Polymerase
Entrez Gene IDs
142 (Human); 11545 (Mouse)
Alternate Names
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1

Product Datasheets

Citations for Human/Mouse PARP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The Molecular Basis of Tight Nuclear Tethering and Inactivation of cGAS
    Authors: B Zhao, P Xu, CM Rowlett, T Jing, O Shinde, Y Lei, AP West, WR Liu, P Li
    Nature, 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. RNA stability regulates human T cell leukemia virus type 1 gene expression in chronically-infected CD4 T cells
    Authors: HC Lin, PJ Simon, RM Ysla, SL Zeichner, G Brewer, AB Rabson
    Virology, 2017;508(0):7-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Conditional deletion of Nbs1 in murine cells reveals its role in branching repair pathways of DNA double-strand breaks.
    Authors: Yang YG, Saidi A, Frappart PO, Barrucand C, Dumon-Jones V, Michelon J, Herceg Z
    EMBO J., 2006;25(23):5527-38.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Distinct promoters mediate constitutive and inducible Bcl-XL expression in malignant lymphocytes.
    Authors: Habens F, Lapham AS, Dallman CL, Pickering BM, Michels J, Marcusson EG, Johnson PW, Packham G
    Oncogene, 2006;26(13):1910-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Transcriptional repressor activating transcription factor 3 protects human umbilical vein endothelial cells from tumor necrosis factor-alpha-induced apoptosis through down-regulation of p53 transcription.
    Authors: Kawauchi J, Zhang C, Nobori K, Hashimoto Y, Adachi MT, Noda A, Sunamori M, Kitajima S
    J. Biol. Chem., 2002;277(41):39025-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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Reviews for Human/Mouse PARP Antibody

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Human/Mouse PARP Antibody
By Anonymous on 05/16/2017
Application: Growing cells - tissue culture Sample Tested: Umbilical vein endothelial cells Species: Human