Human/Mouse PARP Antibody

• Species Reactivity
  Human, Mouse
• Specificity
  Detects human and mouse PARP in Western blots.
• Source
  Monoclonal Rat IgG_2A Clone # 53015
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  E. coli-derived recombinant mouse PARP
  Val71-Pro329
  Accession # NP_031441
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.6 µg/mL
  See below

Western Blot click image to view larger

Detection of Human PARP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated with anti‑Fas and 1 µM staurosporine (STS) for 0 to 4 hours (h; as indicated). PVDF membrane was probed with 0.6 µg/mL of Rat Anti-Human/Mouse PARP Monoclonal Antibody (Catalog # MAB600) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PARP at approximately 116 kDa and the 84 kDa product of PARP cleavage (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot click image to view larger

Detection of Mouse PARP by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line treated with 1 µM staurosporine (STS) for 0 to 4 hours (h; as indicated). PVDF membrane was probed with 0.6 µg/mL of Rat Anti-Human/Mouse PARP Monoclonal Antibody (Catalog # MAB600) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PARP at approximately 116 kDa and the 60-70 kDa bands generated with staurosporine treatment (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Poly [ADP-ribose] Polymerase

• Entrez Gene IDs:
  142 (Human); 11545 (Mouse)

• Alternate Names:
  ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1

FAQs

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