PARP Antibody

R&D Systems | Catalog # AF-600-NA

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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PARP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PARP
Val71-Pro329
Accession # NP_031441

Specificity

Detects human and mouse PARP in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PARP Antibody

Detection of Human PARP antibody by Western Blot.

Detection of Human PARP by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 ng/mL anti-Fas for 24 hours. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Human/Mouse PARP Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PARP at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
PARP antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

PARP in HeLa Human Cell Line.

PARP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human PARP antibody by Simple WesternTM.

Detection of Human PARP by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PARP at approximately 122 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunoprecipitation of Human PARP.

Immunoprecipitation of Human PARP.

Jurkat human acute T cell leukemia cell line was treated with apoptosis inducer anti-Fas for the indicated times. PARP was immunoprecipitated from cell lysates (1 - 2 x 106cells) following incubation with 5 µg Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) for overnight at 4 °C. PARP-antibody complexes were absorbed using Protein G expressing Staph cells (Sigma). Immunoprecipitated PARP was detected by Western blot using 0.4 µg/mL Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA). View our recommended buffer recipes for immunoprecipitation.
Western Blot Shows Human PARP Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human PARP Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and PARP knockout HEK293T cell line (KO). PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PARP at approximately 120 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293Tcell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PARP Antibody

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunoprecipitation

5 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line treated with anti-Fas, see our available Western blot detection antibodies

Knockout Validated

PARP is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in PARP knockout HEK293T cell line.

Simple Western

5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

0.4 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126)

Reviewed Applications

Read 2 reviews rated 4.5 using AF-600-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PARP

PARP, Poly [ADP-ribose] polymerase 1 (PARP1), is a component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Widely expressed. Expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult. Expressed in B-cells that have been induced to switch to various Ig isotypes. PARP interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Entrez Gene IDs

142 (Human); 11545 (Mouse)

Gene Symbol

PARP1

UniProt

NP_031441

Additional PARP Products

Product Documents for PARP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PARP Antibody

For research use only

Customer Reviews for PARP Antibody (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • Human/Mouse PARP Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    PARP Antibody AF-600-NA
  • Human/Mouse PARP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/17/2018
    PARP Antibody AF-600-NA

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Protocols

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FAQs

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