Human/Mouse PARP Antibody AF-600-NA: R&D Systems

Human/Mouse PARP Antibody

(3 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse PARP in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse PARP
    Val71-Pro329
    Accession # NP_031441
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.4 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunoprecipitation
    5 µg/106 cells
    See below
  • Immunocytochemistry
    1-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PARP by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 ng/mL anti-Fas for 24 hours. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Human/Mouse PARP Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PARP at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry
PARP in HeLa Human Cell Line. PARP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunoprecipitation
Immunoprecipitation of Human PARP.

Jurkat human acute T cell leukemia cell line was treated with apoptosis inducer anti-Fas for the indicated times. PARP was immunoprecipitated from cell lysates (1 - 2 x 106 cells) following incubation with 5 µg Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) for overnight at
4 °C. PARP-antibody complexes were absorbed using Protein G expressing Staph cells (Sigma). Immunoprecipitated PARP was detected by Western blot using 0.4 µg/mL Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA). View our recommended buffer recipes for immunoprecipitation.

Detection of Human PARP by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PARP at approximately 122 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse PARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-600-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PARP
PARP, Poly [ADP-ribose] polymerase 1 (PARP1), is a component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Widely expressed. Expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult. Expressed in B-cells that have been induced to switch to various Ig isotypes. PARP interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression.
  • Long Name:
    Poly [ADP-ribose] Polymerase
  • Entrez Gene IDs:
    142 (Human); 11545 (Mouse)
  • Alternate Names:
    ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Nuclear-translocated Glyceraldehyde-3-phosphate Dehydrogenase Promotes Poly(ADP-ribose) Polymerase-1 Activation during Oxidative/Nitrosative Stress in Stroke.
    Authors: Nakajima H, Kubo T, Ihara H, Hikida T, Danjo T, Nakatsuji M, Shahani N, Itakura M, Ono Y, Azuma Y, Inui T, Kamiya A, Sawa A, Takeuchi T
    J Biol Chem, 2015;290(23):14493-503.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Dual targeting of HER2-positive cancer with trastuzumab emtansine and pertuzumab: critical role for neuregulin blockade in antitumor response to combination therapy.
    Authors: Phillips G, Fields C, Li G, Dowbenko D, Schaefer G, Miller K, Andre F, Burris H, Albain K, Harbeck N, Dieras V, Crivellari D, Fang L, Guardino E, Olsen S, Crocker L, Sliwkowski M
    Clin Cancer Res, 2014;20(2):456-68.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Nuclear localization of Survivin renders HeLa tumor cells more sensitive to apoptosis by induction of p53 and Bax.
    Authors: Temme A, Rodriguez JA, Hendruschk S, Gunes S, Weigle B, Schakel K, Schmitz M, Bachmann M, Schackert G, Rieber EP
    Cancer Lett., 2007;250(2):177-93.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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