Human/Mouse Phospho-MSK2/RSKB (S196) Antibody

• Species Reactivity
  Human, Mouse
• Specificity
  Detects human and mouse MSK2/RSKB when phosphorylated at S196.
• Source
  Polyclonal Rabbit IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Phosphopeptide containing human and mouse MSK2/RSKB S196 site
• Formulation
  Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.5 µg/mL
  See below
• Immunohistochemistry
  5-15 µg/mL
  See below

Immunohistochemistry click image to view larger

MSK2/RSKB in Human Pancreas. MSK2/RSKB was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot click image to view larger

Detection of Human Phospho-MSK2/RSKB (S196) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human HGF (Catalog # 294-HG) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Shipping
  The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

• Long Name:
  Mitogen- and Stress-activated Protein Kinase 2

• Entrez Gene IDs:
  8986 (Human); 56613 (Mouse)

• Alternate Names:
  MSK2; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSKB

FAQs

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