Human/Mouse RASSF2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human RASSF2 by Western Blot.
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Product Details
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Human/Mouse RASSF2 Antibody Summary

Species Reactivity
Human, Mouse
Detects endogenous human and mouse RASSF2 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human RASSF2
Accession # P50749
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RASSF2 antibody by Western Blot. View Larger

Detection of Human RASSF2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse RASSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RASSF2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry RASSF2 antibody in U937 Human Cell Line by Immunocytochemistry (ICC). View Larger

RASSF2 in U937 Human Cell Line. RASSF2 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human/Mouse RASSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5639) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RASSF2

RASSF2 (Ras association domain-containing protein 2; also RASSF2A) is a 34‑36 kDa member of the RASSF family of proteins. It is widely expressed, and serves as a tumor suppressor by interacting with K-Ras in a GTP-dependent manner, and activating caspase-3 and inducing apoptosis. Human RASSF2 is 326 amino acids (aa) in length. It contains a bipartite NLS (aa 151‑167), a Ras-association domain (aa 176‑264) and a SARAH domain (aa 272‑319) that mediates homo- and heterotypic interactions. Multiple isoforms exist. One shows an insertion of 13 aa after Lys213, a second shows a three aa substitution for aa 305‑326, and a third shows a 20 aa substitution for aa 1‑96, accompanied by a three aa substitution for aa 231‑326. RASSF2 is often found down regulated in lung-derived tumor cell lines.

Long Name
Ras Association [RalGDS/AF-6] Domain Family Member 2
Entrez Gene IDs
9770 (Human); 215653 (Mouse); 311437 (Rat)
Alternate Names
KIAA0168DKFZp781O1747; Ras association (RalGDS/AF-6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; ras association domain-containing protein 2; RASFADIN; RASSF2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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Human/Mouse RASSF2 Antibody
By Anonymous on 04/06/2022
Application: IHC Sample Tested: Melanoma tissue Species: Human

Tissue was cryopreserved