Detection of Human, Mouse, and Rat ARK5/NUAK1 by Western Blot.
Western blot shows lysates of Saos‑2 human osteosarcoma cell line, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ARK5/NUAK1 Monoclonal Antibody (Catalog # MAB6978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ARK5/NUAK1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
AMPK-related kinase 5 (ARK5), also known
as NUAK1, is a 74 kDa intracellular Ser/Thr kinase that promotes cell survival
during nutrient deprivation and TNF family ligand-induced apoptosis. ARK5 is
activated by Akt, NDR2, and LKB1-mediated phosphorylation at multiple serine
and threonine residues. It inhibits the activation of Caspase-6, Caspase-8, and
myosin phosphatases but promotes the activation of MMP2 and MMP9. ARK5
overexpression in several cancers contributes to increased tumor progression
and aggressiveness. ARK5 contains one catalytic domain (aa 65-306), and a short
isoform of the human protein is prematurely tuncated within this domain. Within
aa 311-462, human ARK5 shares 88% and 85% aa sequence identity with mouse and
rat ARK5, respectively.
AMPK-related Protein Kinase 5
Entrez Gene IDs:
9891 (Human); 77976 (Mouse); 299694 (Rat)
AMPK-related protein kinase 5; ARK5; ARK5AMP-activated protein kinase family member 5; EC 2.7.11; EC 188.8.131.52; KIAA0537NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; NUAK1
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