Human/Mouse/Rat ATG3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat ATG3 by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products

Human/Mouse/Rat ATG3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse and rat ATG3 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human ATG3
Accession # Q9NT62
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat ATG3 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat ATG3 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat ATG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5450) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry ATG3 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

ATG3 in RAW 264.7 Mouse Cell Line. ATG3 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line, untreated (lower panel) or treated with LPS (upper panel), using Sheep Anti-Human/Mouse/Rat ATG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5450) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATG3

ATG3 (autophagy-related protein 3; also APG3-like and PC3-96) is a ubiquitous 45 kDa member of the ATG3 family of proteins. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG7-bound ATG8 (known as LC3, GATE16 and GABA-RAP in mammals) to phosphatidylethanolamine, critical for autophagy. Human ATG3 is 314 amino acids in length and contains an active site at Cys264 that forms a thiol ester bond with the C-terminal Gly of ATG8. There are multiple potential isoform variants. Three show a 22, 28 and 35 aa substitution for aa 289‑314, respectively, while a fourth shows an alternate start site at Met88 that may be accompanied by one of the afore mentioned substitutions. Over aa 1‑287, human ATG3 shares 97.9% aa identity with mouse ATG3.

Long Name
Autophagy Related 3 Homolog
Entrez Gene IDs
64422 (Human); 67841 (Mouse); 171415 (Rat)
Alternate Names
APG3 autophagy 3-like (S. cerevisiae); APG3; APG3L; APG3-LIKE; Apg3p; ATG3 autophagy related 3 homolog (S. cerevisiae); ATG3; Autophagy-related protein 3; DKFZp564M1178; EC 6.3.2.-; FLJ22125,2610016C12Rik; hApg3; MGC15201; PC3-96; Protein PC3-96; ubiquitin-like-conjugating enzyme ATG3

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Citation for Human/Mouse/Rat ATG3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Escitalopram oxalate induces apoptosis in U-87MG cells and autophagy in GBM8401 cells
    Authors: VC Chen, YH Hsieh, LJ Chen, TC Hsu, BS Tzang
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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