Detection of Human/Mouse/Rat ATG3 by Western Blot.
Western blot shows lysates of human brain tissue, mouse brain tissue, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat ATG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5450) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
ATG3 in RAW 264.7 Mouse Cell Line.
ATG3 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line, untreated (lower panel) or treated with LPS (upper panel), using Sheep Anti-Human/Mouse/Rat ATG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5450) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Autophagy Related 3 Homolog
Entrez Gene IDs:
64422 (Human); 67841 (Mouse); 171415 (Rat)
APG3 autophagy 3-like (S. cerevisiae); APG3; APG3L; APG3-LIKE; Apg3p; ATG3 autophagy related 3 homolog (S. cerevisiae); ATG3; Autophagy-related protein 3; DKFZp564M1178; EC 6.3.2.-; FLJ22125,2610016C12Rik; hApg3; MGC15201; PC3-96; Protein PC3-96; ubiquitin-like-conjugating enzyme ATG3
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