Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 384202
View all Bcl-10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bcl-10
Met1-Leu192
Accession # O95999

Specificity

Detects endogenous human, mouse, and rat Bcl-10 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Bcl‑10 Antibody

Detection of Human/Mouse/Rat Bcl‑10 antibody by Western Blot.

Detection of Human/Mouse/Rat Bcl‑10 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Bcl-10 Monoclonal Antibody (Catalog # MAB2889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl-10 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat Bcl‑10 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Bcl-10

B cell CLL/leukemia 10 (Bcl-10), a 233 amino acid, 26.2 kDa protein, is the cellular homolog of the equine herpesvirus-2 gene (E10). Bcl-10 is also known as CARD like apoptotic protein (CLAP), CED-3/ICH-1 prodomain homologous E10-like regulator (CIPER), celluar-E10 (c-E10), and CARD-containing molecule enhancing NF kappa B (CARMEN). Bcl-10 contains a caspase recruitment domain (CARD) and has been shown to induce apoptosis and to activate NF kappa B. Bcl-10 can interact with other CARD domain containing proteins including CARD9, 10, 11, and 14, which function as upstream regulators of NF kappa B signaling. Defects in Bcl‑10 may be involved in the pathogenesis of human malignancy.  Over aa 1-192, human Bcl-10 is 92% and 93% aa identical to mouse and rat Bcl-10, respectively.

Long Name

B Cell Lymphoma/Leukemia 10

Alternate Names

Bcl10, c-E10, CARMEN, CIPER, CLAP, mE10

Entrez Gene IDs

8915 (Human)

Gene Symbol

BCL10

UniProt

O95999

Additional Bcl-10 Products

Product Documents for Human/Mouse/Rat Bcl‑10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Bcl‑10 Antibody

For research use only

Customer Reviews for Human/Mouse/Rat Bcl‑10 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Bcl‑10 Antibody and earn rewards!

Have you used Human/Mouse/Rat Bcl‑10 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies