Human/Mouse/Rat Calcineurin B Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Calcineurin B in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Calcineurin B
    Accession # P63098
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    2.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat Calcineurin B by Western Blot. Western blot shows lysates of mouse brain tissue,
HT‑29 human colon adenocarcinoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat Calcineurin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1348) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Calcineurin B at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Calcineurin B by Simple WesternTM. Simple Western lane view shows lysates of HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Calcineurin B at approximately 24 kDa (as indicated) using 2.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Calcineurin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1348). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calcineurin B

Calcineurin, also called Protein Phosphatase 2B, PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59,000 dalton catalytic A subunit and a 1,900 dalton regulatory B subunit that is activated by the binding of calcium ions and calmodulin (1). Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory (2). It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation (3). Complexes of the immunosuppressants cyclosporin and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity (4). Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy (5) and graft versus host disease in organ transplantation (6).

  • References:
    1.  Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
    2. Zeng H. et al. (2001) Cell 107:617.
    3. Okamura, H. et al. (2000) Molecular Cell 6:539.
    4. Liu, J. et al. (1992) Biochemistry 31:3896.
    5. Molkentin, J.D. (2000) Circulation Research 87:731.
    6. Sanquer, S.A. et al. (2004) Transplantation 77:854.
  • Entrez Gene IDs:
    5534 (Human); 19058 (Mouse); 29748 (Rat)
  • Alternate Names:
    Calcineurin B; calcineurin subunit B type 1; CALNB1; CNA2; Cnb1; MCIP1; PP2B beta 1; PPP3R1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha; type I (19kDa)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Regulation of RCAN1 protein activity by Dyrk1A protein-mediated phosphorylation.
    Authors: Jung MS, Park JH, Ryu YS, Choi SH, Yoon SH, Kwen MY, Oh JY, Song WJ, Chung SH
    J. Biol. Chem., 2011;286(46):40401-12.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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