Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Hamster

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Calcineurin B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Calcineurin B
Accession # P63098

Specificity

Detects human, mouse, and rat Calcineurin B in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Calcineurin B Antibody

Detection of Human/Mouse/Rat Calcineurin B antibody by Western Blot.

Detection of Human/Mouse/Rat Calcineurin B by Western Blot.

Western blot shows lysates of mouse brain tissue, HT-29 human colon adenocarcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat Calcineurin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1348) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Calcineurin B at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Calcineurin B antibody by Simple WesternTM.

Detection of Human Calcineurin B by Simple WesternTM.

Simple Western lane view shows lysates of HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Calcineurin B at approximately 24 kDa (as indicated) using 2.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Calcineurin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1348). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Calcineurin B Antibody

Application
Recommended Usage

Simple Western

2.5 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line

Western Blot

0.25 µg/mL
Sample: Mouse brain tissue, HT-29 human colon adenocarcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and TS1 mouse helper T cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calcineurin B

Calcineurin, also called Protein Phosphatase 2B, PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59,000 dalton catalytic A subunit and a 1,900 dalton regulatory B subunit that is activated by the binding of calcium ions and calmodulin (1). Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory (2). It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation (3). Complexes of the immunosuppressants cyclosporin and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity (4). Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy (5) and graft versus host disease in organ transplantation (6).

References

  1.  Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
  2. Zeng H. et al. (2001) Cell 107:617.
  3. Okamura, H. et al. (2000) Molecular Cell 6:539.
  4. Liu, J. et al. (1992) Biochemistry 31:3896.
  5. Molkentin, J.D. (2000) Circulation Research 87:731.
  6. Sanquer, S.A. et al. (2004) Transplantation 77:854.

Alternate Names

CALNB1, Cnb1, MCIP1, PP2B beta 1, PPP3R1

Entrez Gene IDs

5534 (Human); 19058 (Mouse); 29748 (Rat)

Gene Symbol

PPP3R1

UniProt

P63098

Additional Calcineurin B Products

Product Documents for Human/Mouse/Rat Calcineurin B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Calcineurin B Antibody

For research use only

Citations for Human/Mouse/Rat Calcineurin B Antibody

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