Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Casein Kinase 1 delta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CK1 delta
Arg316-Arg415
Accession # P48730

Specificity

Detects endogenous human, mouse and rat CK1 delta in Western blots. In Western blots, this antibody does not cross‑react with recombinant human CK1 alpha or rhCK1 gamma ; reactivity with CK1 epsilon is unknown.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Casein Kinase 1 delta Antibody

Detection of Human/Mouse/Rat Casein Kinase 1d antibody by Western Blot.

Detection of Human/Mouse/Rat Casein Kinase 1 delta by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1d at approximately 49 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Casein Kinase 1d antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

Casein Kinase 1 delta in C2C12 Mouse Cell Line.

Casein Kinase 1d was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse/Rat Casein Kinase 1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Casein Kinase 1 delta Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line

Western Blot

1 µg/mL
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and Y3-Ag rat myeloid cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Casein Kinase 1 delta

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3 , delta  and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 delta phosphorylates p53 as well as MDM2 and seems to play a principal role in coordinating the effects of these two key regulator proteins on cell growth and genome integrity.

Alternate Names

CSNK1D, HCKID

Entrez Gene IDs

1453 (Human); 104318 (Mouse); 64462 (Rat)

Gene Symbol

CSNK1D

UniProt

P48730

Additional Casein Kinase 1 delta Products

Product Documents for Human/Mouse/Rat Casein Kinase 1 delta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Casein Kinase 1 delta Antibody

For research use only

Related Research Areas

Intracellular Kinases

Citations for Human/Mouse/Rat Casein Kinase 1 delta Antibody

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Protocols

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